Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Division, National Research Centre, Dokki, Giza, 12622, Egypt.
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.
Biol Trace Elem Res. 2021 Jul;199(7):2788-2799. doi: 10.1007/s12011-020-02369-4. Epub 2020 Sep 8.
Mycogenic synthesis of medically applied zinc oxide (ZnO) and copper oxide (CuO) nanoparticles (NPs) were exploited using Penicillium chrysogenum. The biogenesis and capping processes of the produced nano-metals were conducted by functional fungal extracellular enzymes and proteins. The obtained ZnO-NPs and CuO-NPs were characterized. Also, the antibacterial activity and minimum inhibitory concentration (MIC) values of ZnO-NPs and CuO-NPs were determined. Also, antibiofilm and antifungal activities were investigated. Results have demonstrated the ability of the bio-secreted proteins to cape and reduce ZnO and CuO to hexagonal and spherical ZnO-NPs and CuO-NPs with particle size at 9.0-35.0 nm and 10.5-59.7 nm, respectively. Both ZnO-NPs and CuO-NPs showed high antimicrobial activities not only against Gram-positive and Gram-negative bacteria but also against some phytopathogenic fungal strains. Besides this, those NPs showed varied antibiofilm effects against different microorganisms. Quantitative and qualitative analyses indicated that CuO-NPs had an effective antibiofilm activity against Staphylococcus aureus and therefore can be applied in diverse medical devices. Thus, the mycogenic green synthesized ZnO-NPs and CuO-NPs have the potential as smart nano-materials to be used in the medical field to limit the spread of some pathogenic microbes.
采用青霉属真菌（Penicillium chrysogenum）进行了医用氧化锌（ZnO）和氧化铜（CuO）纳米粒子（NPs）的真菌合成。所产生的纳米金属的生物发生和封端过程是通过功能性真菌细胞外酶和蛋白质进行的。对获得的 ZnO-NPs 和 CuO-NPs 进行了表征。还测定了 ZnO-NPs 和 CuO-NPs 的抗菌活性和最小抑菌浓度（MIC）值。还研究了抗生物膜和抗真菌活性。结果表明，生物分泌的蛋白质能够将 ZnO 和 CuO 包覆并还原为六方和球形的 ZnO-NPs 和 CuO-NPs，粒径分别为 9.0-35.0nm 和 10.5-59.7nm。ZnO-NPs 和 CuO-NPs 均表现出对革兰氏阳性菌和革兰氏阴性菌以及一些植物病原菌真菌株的高抗菌活性。除此之外，这些 NPs 对不同的微生物表现出不同的抗生物膜作用。定量和定性分析表明，CuO-NPs 对金黄色葡萄球菌具有有效的抗生物膜活性，因此可应用于各种医疗器械。因此，真菌绿色合成的 ZnO-NPs 和 CuO-NPs 具有作为智能纳米材料在医学领域应用的潜力，以限制一些致病性微生物的传播。
