College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Medical School, Southeast University, Nanjing, 210009, China.
Chemosphere. 2022 Aug;301:134687. doi: 10.1016/j.chemosphere.2022.134687. Epub 2022 Apr 23.
With the increase in application, multi-walled carbon nanotubes (MWCNTs) are potentially bioavailable to environmental organisms. However, the potential transgenerational effect of MWCNTs and underlying mechanisms remains still unclear. Here, we examined transgenerational MWCNT toxicity and the underlying mechanism mediated by germline long non-coding RNAs (lncRNAs) in Caenorhabditis elegans. Exposure to 0.1-10 μg/L MWCNT caused transgenerational toxicity reflected by endpoints of brood size and locomotion behavior. Meanwhile, among germline lncRNAs, expression of 5 lncRNAs were dysregulated by MWCNT exposure. Among these 5 dysregulated lncRNAs, only germline RNAi of linc-7 affected MWCNT toxicity. Increase in germline linc-7 expression was observed transgenerationally, and transgenerational MWCNT toxicity was prevented in linc-7(RNAi) nematodes. Moreover, germline linc-7 controlled transgenerational MWCNT toxicity by activating downstream DAF-12, a transcriptional factor. Therefore, our data indicated the association between induction of transgenerational MWCNT toxicity and increase in germline linc-7 expression in organisms.
随着应用的增加，多壁碳纳米管（MWCNTs）有可能对环境生物具有生物利用度。然而，MWCNTs 的潜在跨代效应及其潜在机制仍不清楚。在这里，我们在秀丽隐杆线虫中检查了由生殖系长非编码 RNA（lncRNA）介导的跨代 MWCNT 毒性及其潜在机制。暴露于 0.1-10μg/L MWCNT 导致代际毒性，表现在种群大小和运动行为等终点上。同时，在生殖系 lncRNA 中，有 5 个 lncRNA 的表达被 MWCNT 暴露所调控。在这 5 个失调的 lncRNA 中，只有 linc-7 的生殖系 RNAi 会影响 MWCNT 的毒性。观察到生殖系 linc-7 的表达在代际间增加，并且 linc-7（RNAi）线虫中的跨代 MWCNT 毒性得到了预防。此外，生殖系 linc-7 通过激活下游转录因子 DAF-12 来控制跨代 MWCNT 毒性。因此，我们的数据表明，在生物体中，诱导跨代 MWCNT 毒性与生殖系 linc-7 表达的增加之间存在关联。
