Opt Express. 2022 Mar 28;30(7):10456-10469. doi: 10.1364/OE.444796.
Glass fibers are miniature optical components that serve as ultra-narrow endoscopy probes. Ideally, one would want to perform imaging through a fiber at the highest achievable resolution and speed. State-of-the-art super-resolution techniques have shattered the diffraction limit, but more than twofold improvement requires fluorescent labeling and a long acquisition time. Moreover, it is challenging to implement super-resolution microscopy in a fiber format. Here we present fiber-based label-free video-rate imaging at more than 2-fold higher resolution than the diffraction limit. Our work paves the way to rapid, sub-wavelength endo-microscopy in unlabeled live specimens.
玻璃纤维是微型光学元件，可用作超窄内窥镜探头。理想情况下，人们希望通过光纤以最高可达的分辨率和速度进行成像。最先进的超分辨率技术已经突破了衍射极限，但要提高两倍以上的分辨率，需要荧光标记和较长的采集时间。此外，在光纤格式中实现超分辨率显微镜技术具有挑战性。在这里，我们展示了基于光纤的无标记视频速率成像，其分辨率比衍射极限高出两倍以上。我们的工作为在未经标记的活标本中进行快速、亚波长的内窥显微镜铺平了道路。
