Redha Mahdi A, Al Sweih Noura, Albert M John
Department of Microbiology, Faculty of Medicine, Kuwait University, Jabriya, Kuwait.
Gut Pathog. 2022 Apr 26;14(1):18. doi: 10.1186/s13099-022-00490-4.
In Kuwait, some untreated sewage is discharged into the sea which poses health risks. Therefore, we determined the virulence traits and the phylogenetic groups of E. coli cultured from raw sewage. Sewage was collected once every month for 12 months with culturing of a total of 140 E. coli isolates. E. coli was typed by the methods of Clermont. The five pathotypes of diarrheagenic E. coli (DEC), and extra-intestinal pathogenic E. coli (ExPEC) were detected by specific PCR assays. Four virulence genes which correlate with pathogenicity in animal models, were used for the first time for detection of ExPEC-vat (vacuolating auto-transporter toxin), fyuA (yersiniabactin receptor), chuA (heme-binding protein), and yfcV (major subunit of putative chaperon-usher fimbria). Most E. coli belonged to phylogenetic groups A (65[45%]) and B1 (34[24.3%]). Three (2.1%) isolates were DEC, while 14 (10%) isolates were ExPEC mostly in group B2 (57.1%). A relatively high prevalence of ExPEC in sewage has public health implications.
在科威特，一些未经处理的污水被排放到海里，这带来了健康风险。因此，我们测定了从未经处理的污水中培养出的大肠杆菌的毒力特征和系统发育群。连续12个月每月采集一次污水，共培养出140株大肠杆菌分离株。采用克莱蒙特方法对大肠杆菌进行分型。通过特异性PCR检测方法检测了致泻性大肠杆菌（DEC）的五种致病型以及肠外致病性大肠杆菌（ExPEC）。首次使用与动物模型致病性相关的四个毒力基因来检测ExPEC——vat（空泡化自转运毒素）、fyuA（耶尔森菌素受体）、chuA（血红素结合蛋白）和yfcV（假定的伴侣-usher菌毛的主要亚基）。大多数大肠杆菌属于系统发育群A（65株[45%]）和B1（34株[24.3%]）。三株（2.1%）分离株为DEC，而14株（10%）分离株为ExPEC，大多在B2群（57.1%）。污水中ExPEC的相对高流行率具有公共卫生意义。
