Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
Anal Chim Acta. 2022 May 8;1206:339809. doi: 10.1016/j.aca.2022.339809. Epub 2022 Apr 4.
Lanthanide coordination polymer nanoparticles (LML-Eu-GMP CPNPs) were fabricated as a ratiometric fluorescence sensor for real-time and visual assays of tetracycline (TC). The LML-Eu-GMP CPNPs were prepared by spontaneous self-assembly of Eu, luminol (LML) and guanosine 5'-monophosphate (GMP) at room temperature, which only emitted blue fluorescence dominated by LML, and no red characteristic fluorescence of Eu was observed. In the presence of TC, TC can trigger the antenna effect (AE) and inner filter effect (IFE), the blue fluorescence of LML-Eu-GMP CPNPs is quenched by TC via IFE, and the red characteristic fluorescence of Eu can be turned on through AE. When sodium citrate (Cit) is added to the sensing system, it can further enhance the red fluorescence of Eu. The dual-ligand CPNP sensor has a rapid fluorescence response, noticeable fluorescence color change, good selectivity, and high sensitivity with a detection limit of 3.4 nM. It was successfully applied to detect TC in honey, milk, lake water and tap water. Meanwhile, the portable smartphone and test paper were applied to develop POCT devices for real-time and visual detection of TC, which could offer a promising application for real-time and semiquantitative assays of TC.
镧系配合物聚合物纳米粒子（LML-Eu-GMP CPNPs）被制备为比率荧光传感器，用于四环素（TC）的实时和可视化测定。LML-Eu-GMP CPNPs 通过 Eu、发光体（LML）和鸟苷 5'-单磷酸（GMP）在室温下自发自组装制备，仅发射由 LML 主导的蓝色荧光，并且观察不到 Eu 的红色特征荧光。在 TC 的存在下，TC 可以触发天线效应（AE）和内滤效应（IFE），通过 IFE，TC 猝灭 LML-Eu-GMP CPNPs 的蓝色荧光，并且通过 AE 可以打开 Eu 的红色特征荧光。当向传感体系中加入柠檬酸钠（Cit）时，它可以进一步增强 Eu 的红色荧光。双配体 CPNP 传感器具有快速的荧光响应、明显的荧光颜色变化、良好的选择性和高灵敏度，检测限为 3.4 nM。它成功地应用于蜂蜜、牛奶、湖水和自来水中 TC 的检测。同时，便携式智能手机和试纸被应用于开发用于 TC 的实时和可视化检测的 POCT 设备，这为 TC 的实时和半定量分析提供了有前景的应用。
