School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, People's Republic of China.
Tai'an Center for Disease Control and Prevention, Tai'an, 271000, People's Republic of China.
Mikrochim Acta. 2023 Aug 16;190(9):354. doi: 10.1007/s00604-023-05933-6.
Curcumin (Cur) possesses diverse biological and pharmacologic effects. It is widely used as a food additive and therapeutic medicine. A study to determine a sensitive detection method for Cur is necessary and meaningful. In this work, double rare earth ions co-doped fluorescent coordination polymer nanoparticles (CPNPs) were developed for the Cur detection. The CPNPs were synthesized by using adenosine monophosphate (AMP) as bridge ligands via coordination self-assembly with Ce and Tb. The AMP-Ce/Tb CPNPs exhibited the characteristic green fluorescence of Tb and had high luminescence efficiency. Under the optimal conditions, the fluorescence intensity of AMP-Ce/Tb CPNPs could be significantly quenched by Cur. The fluorescence quenching extent at λ/λ of 300 nm/544 nm showed a good linear relationship with the Cur concentration in the range of 10 to 1000 nM. The detection limit was as low as 8.0 nM (S/N = 3). This method was successfully applied to the determination of Cur in real samples with satisfactory results. The luminescence mechanism of AMP-Ce/Tb CPNPs and the fluorescence quenching mechanism of the CPNPs by Cur were both examined.
姜黄素（Cur）具有多种生物学和药理学作用。它被广泛用作食品添加剂和治疗药物。因此，有必要开发一种用于姜黄素检测的灵敏检测方法。在这项工作中，我们开发了双稀土离子共掺杂荧光配位聚合物纳米粒子（CPNPs）用于姜黄素的检测。通过与铈和铽的配位自组装，以单磷酸腺苷（AMP）作为桥联配体合成了 CPNPs。AMP-Ce/Tb CPNPs 表现出铽的特征绿光，并且具有高光致发光效率。在最佳条件下，AMP-Ce/Tb CPNPs 的荧光强度可被姜黄素显著猝灭。在λ/λ为 300nm/544nm 时，荧光猝灭程度与 10 至 1000nM 范围内的姜黄素浓度呈良好的线性关系。检测限低至 8.0 nM（S/N = 3）。该方法成功地应用于实际样品中姜黄素的测定，结果令人满意。还研究了 AMP-Ce/Tb CPNPs 的发光机制以及 CPNPs 被姜黄素荧光猝灭的机制。
