Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, Dak Lak, 630000, Vietnam.
School of Economics and Management, Far Eastern Federal University, Vladivostok, 690922, Russia.
Mol Biol Rep. 2022 May;49(5):4141-4148. doi: 10.1007/s11033-022-07471-5. Epub 2022 Apr 26.
Bacillus velezensis possesses numerous chitinolytic enzymes; however, not much is known about the role of its chitinase molecules.
In this study, the chiA gene, which encodes a novel domain structure possessing family 18 chitinase from B. velezensis RB.IBE29, was expressed successfully in Escherichia coli BL21-CodonPlus (DE3)-RIPL using the pColdII expression vector. The recombinant protein, rBvChiA, was purified using the HisTrap FF column. Purified rBvChiA showed hydrolytic activity against insoluble chitin and bound to chitinous substrates. In addition, the purified recombinant enzyme displayed remarkable inhibition effects on the spore germination of Fusarium falciforme and the egg hatch of root-knot nematodes (Meloidogyne spp.), which are the main causes of black pepper diseases in the Central Highlands region, Vietnam.
The current work results might enable further studies to develop novel chitinase A and strain RB.IBE29 as a natural fungicide and nematicide for sustainable black pepper production and other crops in the Central Highlands, Vietnam. This is the second report describing chitinase from B. velezensis based on the experimental data.
解淀粉芽孢杆菌拥有多种几丁质酶；然而，其几丁质酶分子的作用知之甚少。
在这项研究中，成功地在大肠杆菌 BL21-CodonPlus（DE3）-RIPL 中使用 pColdII 表达载体表达了来自解淀粉芽孢杆菌 RB.IBE29 的编码具有家族 18 几丁质酶的新型结构域的 chiA 基因。使用 HisTrap FF 柱纯化重组蛋白 rBvChiA。纯化的 rBvChiA 对不溶性几丁质具有水解活性，并与几丁质底物结合。此外，纯化的重组酶对越南中高原地区黑胡椒病害的主要病原菌镰孢菌孢子萌发和根结线虫（Meloidogyne spp.）卵孵化具有显著抑制作用。
目前的工作结果可能使进一步的研究能够开发新型几丁质酶 A 和菌株 RB.IBE29，作为可持续黑胡椒生产和越南中高原其他作物的天然杀菌剂和杀线虫剂。这是根据实验数据描述解淀粉芽孢杆菌几丁质酶的第二份报告。
