Faculty of Science, Hokkaido University, Sapporo, Japan.
Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, Japan.
Methods Mol Biol. 2022;2430:47-59. doi: 10.1007/978-1-0716-1983-4_3.
The filamentous cytoskeletal protein microtubule, a polymer of α and β heterodimers of tubulin, plays major roles in intracellular transport as well as in vitro molecular actuation and transportation. Functionalization of tubulin dimers through covalent linkage facilitates utilization of microtubule in the nanobioengineering. Here we present a detailed description of the methodologies used to modify tubulin dimers with DNA strand and biotin through covalent interaction.
丝状细胞骨架蛋白微管是由α和β微管蛋白异二聚体组成的聚合物，在细胞内运输以及体外分子驱动和运输中发挥主要作用。通过共价键连接对微管蛋白二聚体进行功能化，有助于在纳米生物工程中利用微管。本文详细介绍了通过共价相互作用用 DNA 链和生物素修饰微管蛋白二聚体的方法。
