Akter Mousumi, Kabir Arif Md Rashedul, Keya Jakia Jannat, Sada Kazuki, Asanuma Hiroyuki, Kakugo Akira
Department of Mechanical Engineering, University of Michigan, Ann Arbor, 48108, Michigan United States.
Faculty of Science, Hokkaido University, Sapporo, 060-0810, Japan.
ACS Omega. 2024 Aug 23;9(36):37748-37753. doi: 10.1021/acsomega.4c03216. eCollection 2024 Sep 10.
The swarming of self-propelled cytoskeletal filaments has emerged as a new aspect in the field of molecular machines or robotics, as it has overcome one of the major challenges of controlling the mutual interaction of a large number of individuals at a time. Recently, we reported on the photoregulated swarming of kinesin-driven cytoskeletal microtubule filaments in which visible (VIS) and ultraviolet (UV) light triggered the association and dissociation of the swarm, respectively. However, systematic control of this potential system has yet to be achieved to optimize swarming for further applications in molecular machines or robotics. Here, we demonstrate the precise and localized control of a biomolecular motor-based swarm system by varying different parameters related to photoirradiation. We control the reversibility of the swarming by changing the wavelength or intensity of light and the number of azobenzenes in DNA. In addition, we regulate the swarming in local regions by introducing different-sized or shaped patterns in the UV light system. Such a detailed study of the precise control of swarming would provide new perspectives for developing a molecular swarm system for further applications in engineering systems.
自驱动细胞骨架细丝的群体行为已成为分子机器或机器人领域的一个新方向，因为它克服了一次控制大量个体之间相互作用的一个主要挑战。最近，我们报道了驱动蛋白驱动的细胞骨架微管细丝的光调控群体行为，其中可见光（VIS）和紫外光（UV）分别触发了群体的聚集和解离。然而，为了在分子机器或机器人领域进一步应用而优化群体行为，对这个潜在系统的系统控制尚未实现。在这里，我们通过改变与光照射相关的不同参数，展示了对基于生物分子马达的群体系统的精确和局部控制。我们通过改变光的波长或强度以及DNA中偶氮苯的数量来控制群体行为的可逆性。此外，我们通过在紫外光系统中引入不同尺寸或形状的图案来调节局部区域的群体行为。对群体行为精确控制的如此详细的研究将为开发用于工程系统进一步应用的分子群体系统提供新的视角。
