Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki, Japan.
Int J Syst Evol Microbiol. 2022 Apr;72(4). doi: 10.1099/ijsem.0.005347.
A neutrophilic iron-oxidizing bacterium, strain MIZ01, which was previously isolated from a wetland in Ibaraki, Japan, was taxonomically characterized in detail. Strain MIZ01 was a motile, curved-rod shaped, Gram-stain-negative bacterium. It was able to grow at 10-40 °C (optimally at 30-35 °C) and at pH 5.5-7.0 (optimally at pH 6.0). It grew microaerobically and chemolithoautotrophically using thiosulfate, in addition to ferrous iron, as the sole electron donor. Major cellular fatty acids of strain MIZ01 were C 7/C 6 and C. The complete genome sequence (2.74 Mbp) was determined, showing that its DNA G+C content was 60.0 mol%. Phylogenetic analyses indicated that strain MIZ01 belonged to the family , class , and was closely related to an isolate tentatively named '' ES-1 (98.2 % of 16S rRNA gene sequence similarity). Based on its phenotypic and phylogenetic characteristics, we conclude that strain MIZ01 represents a new genus and species in the family for which we propose the name gen. nov., sp. nov. The type strain is strain MIZ01 (=JCM 39089=DSM 111897).
从日本茨城县的湿地中分离到一株嗜中性铁氧化细菌，菌株 MIZ01，对其进行了详细的分类学特征研究。MIZ01 菌株是一种能动的、弯曲杆状、革兰氏阴性细菌。它能够在 10-40°C（最佳温度为 30-35°C）和 pH5.5-7.0（最佳 pH6.0）的条件下生长。它在微氧和化能自养条件下，以硫代硫酸盐和亚铁离子作为唯一电子供体进行生长。MIZ01 菌株的主要细胞脂肪酸为 C7/C6 和 C。该菌株的完整基因组序列（2.74 Mbp）已被确定，其 DNA G+C 含量为 60.0 mol%。系统发育分析表明，MIZ01 菌株属于 科，类，与暂定名为“ES-1”的分离株（16S rRNA 基因序列相似度为 98.2%）密切相关。基于其表型和系统发育特征，我们认为 MIZ01 菌株代表了一个新的属和种，我们建议将其命名为“Neutrospira gen. nov.，sp. nov.”。模式菌株为 MIZ01 菌株（=JCM 39089=DSM 111897）。
