German Center for Neurodegenerative Diseases (DZNE), 53127 Bonn, Germany.
German Center for Neurodegenerative Diseases (DZNE), 53127 Bonn, Germany; Frankfurt Institute for Advanced Studies, 60438 Frankfurt am Main, Germany; Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with the Max Planck Society, 60528 Frankfurt am Main, Germany.
Cell Rep. 2022 Apr 26;39(4):110746. doi: 10.1016/j.celrep.2022.110746.
The cytoskeleton is crucial for defining neuronal-type-specific dendrite morphologies. To explore how the complex interplay of actin-modulatory proteins (AMPs) can define neuronal types in vivo, we focused on the class III dendritic arborization (c3da) neuron of Drosophila larvae. Using computational modeling, we reveal that the main branches (MBs) of c3da neurons follow general models based on optimal wiring principles, while the actin-enriched short terminal branches (STBs) require an additional growth program. To clarify the cellular mechanisms that define this second step, we thus concentrated on STBs for an in-depth quantitative description of dendrite morphology and dynamics. Applying these methods systematically to mutants of six known and novel AMPs, we revealed the complementary roles of these individual AMPs in defining STB properties. Our data suggest that diverse dendrite arbors result from a combination of optimal-wiring-related growth and individualized growth programs that are neuron-type specific.
细胞骨架对于定义神经元类型特有的树突形态至关重要。为了探索肌动蛋白调节蛋白（AMPs）的复杂相互作用如何在体内定义神经元类型，我们专注于果蝇幼虫的 III 类树突状分支（c3da）神经元。使用计算建模，我们揭示了 c3da 神经元的主要分支（MBs）遵循基于最佳布线原理的一般模型，而富含肌动蛋白的短终端分支（STBs）需要额外的生长程序。为了阐明定义这第二步的细胞机制，因此我们专注于 STBs，对树突形态和动力学进行深入的定量描述。我们将这些方法系统地应用于六个已知和新的 AMPs 的突变体，揭示了这些单个 AMP 在定义 STB 特性方面的互补作用。我们的数据表明，不同的树突分支是由与最佳布线相关的生长和神经元类型特异性的个体化生长程序的组合产生的。
