Jia Yufeng, Hu Yingxin
School of Economics and Management, Shijiazhuang Tiedao University Shijiazhuang 050043 P. R. China.
College of Information Science and Technology, Shijiazhuang Tiedao University Shijiazhuang 050043 P. R. China
RSC Adv. 2021 Sep 10;11(48):30377-30382. doi: 10.1039/d1ra05242j. eCollection 2021 Sep 6.
Toehold-mediated strand displacement is widely used to construct and operate DNA nanodevices. Cooperative regulation of strand displacement with diverse factors is pivotal in the design and construction of functional and dynamic devices. Herein, a cofactor-assisted three-way DNA junction-driven strand displacement strategy was reported, which could tune the reaction kinetics by the collaboration of DNA and other types of stimulus. This strategy is responsive to various inputs by incorporation of the specific sequence into the three-way junction structure. Specifically, the cooperation of multiple factors changes the conformation of the specific domain and promotes the reaction. To demonstrate the strategy, adenosine triphosphate (ATP), HG, and pH were used as cofactors to modulate the displacement reaction. The electrophoresis and fluorescence experiments showed that the cooperative regulation of the strand displacement reaction could be achieved by diverse factors using this strategy. The proposed strategy provides design flexibility for dynamic DNA devices and may have potential in biosensing and biocomputing.
引发链介导的链置换被广泛用于构建和操作DNA纳米器件。将链置换与多种因素进行协同调控在功能性和动态器件的设计与构建中至关重要。在此，报道了一种辅因子辅助的三向DNA连接体驱动的链置换策略，该策略可通过DNA与其他类型刺激的协同作用来调节反应动力学。通过将特定序列掺入三向连接体结构中，此策略可对各种输入做出响应。具体而言，多种因素的协同作用改变了特定结构域的构象并促进了反应。为了证明该策略，使用三磷酸腺苷（ATP）、汞离子（HG）和pH作为辅因子来调节置换反应。电泳和荧光实验表明，利用该策略可通过多种因素实现链置换反应的协同调控。所提出的策略为动态DNA器件提供了设计灵活性，并且可能在生物传感和生物计算方面具有潜力。
