Qu Qi, Wang Jing, Zeng Chuan, Wang Mengfan, Qi Wei, He Zhimin
School of Chemical Engineering and Technology, State Key Laboratory of Chemical Engineering, Tianjin University Tianjin 300350 P. R. China
Technical Center of Zhuhai Entry-Exit Inspection and Quarantine Bureau Zhuhai P. R. China.
RSC Adv. 2021 Jun 28;11(37):22744-22750. doi: 10.1039/d1ra02404c. eCollection 2021 Jun 25.
Owing to the high sensitivity, fast responsiveness and high specificity, immunoassays using surface-enhanced Raman scattering (SERS) as the readout signal displayed great potential in disease diagnosis. In this study, we developed a SERS-immunoassay method for the detection of human immunoglobulin G (HIgG). Upon involving well-ordered AuA on a SERSIA substrate, the LSPR effect was further enhanced to generate a strong and uniform Raman signal through the formation of sandwich structure with the addition of target HIgG and SERSIA tag. Optimization of the assay provided a wide linear range (0.1-200 μg mL) and low limit of detection (0.1 μg mL). In addition, the SERS-immunoassay method displayed excellent specificity and was homogeneous, which guaranteed the practical use of this method in the quantitative detection of HIgG. To validate this assay, human serum was analysed, which demonstrated the potential advantages of SERS-immunoassay technology in clinical diagnostics.
由于具有高灵敏度、快速响应性和高特异性，以表面增强拉曼散射（SERS）作为读出信号的免疫测定在疾病诊断中显示出巨大潜力。在本研究中，我们开发了一种用于检测人免疫球蛋白G（HIgG）的SERS免疫测定方法。在SERSIA基底上引入有序的AuA后，通过加入目标HIgG和SERSIA标签形成夹心结构，进一步增强了局域表面等离子体共振（LSPR）效应，以产生强烈且均匀的拉曼信号。该测定方法的优化提供了宽线性范围（0.1 - 200 μg/mL）和低检测限（0.1 μg/mL）。此外，SERS免疫测定方法具有出色的特异性且为均相测定，这保证了该方法在HIgG定量检测中的实际应用。为验证该测定方法，对人血清进行了分析，这证明了SERS免疫测定技术在临床诊断中的潜在优势。
