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长链鞘磷脂会从不对称脂质膜的细胞质小叶中消耗胆固醇。

Long chain sphingomyelin depletes cholesterol from the cytoplasmic leaflet in asymmetric lipid membranes.

作者信息

Karlsen Maria Lyngby, Bruhn Dennis S, Pezeshkian Weria, Khandelia Himanshu

机构信息

PHYLIFE: Physical Life Science, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark Campusvej 55 Odense 5230 M Denmark

出版信息

RSC Adv. 2021 Jun 28;11(37):22677-22682. doi: 10.1039/d1ra01464a. eCollection 2021 Jun 25.

DOI:10.1039/d1ra01464a
PMID:35480443
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9034350/
Abstract

The transbilayer distribution of cholesterol (CHL) in complex asymmetric lipid membranes remains controversial, with contrasting investigations suggesting that there is more CHL either in the exoplasmic, outer leaflet (OL) or the cytoplasmic, inner leaflet (IL) depending on cell type or model, membrane composition, and method of investigation. Here, we launch systematic coarse-grained molecular dynamics simulations to investigate the impact of the sphingomyelin (SM) acyl chain length upon CHL distribution in asymmetric lipid membrane mixtures which account for the variation of the most abundant headgroups and acyl chain unsaturation in the two membrane leaflets. We find that there is always more CHL in the OL, but longer chain SM depletes more CHL from the IL than short chain SM in simple membrane mixtures containing SM and 16 : 0, 18 : 1 phospholipids. The difference between longer and shorter chain SM is neutralised in a more complex asymmetric membrane, where there are more saturated tails in the outer leaflet. We propose that interdigitation of long-chain SM into the opposing IL pushes cytoplasmic CHL towards the OL, but higher chain saturation of the outer leaflet compensates for the effect of SM chain length.

摘要

胆固醇（CHL）在复杂的不对称脂质膜中的跨膜分布仍存在争议，相互矛盾的研究表明，根据细胞类型或模型、膜组成和研究方法的不同，外质外侧小叶（OL）或细胞质内侧小叶（IL）中存在更多的CHL。在此，我们开展了系统的粗粒度分子动力学模拟，以研究鞘磷脂（SM）酰基链长度对不对称脂质膜混合物中CHL分布的影响，该混合物考虑了两个膜小叶中最丰富的头部基团和酰基链不饱和度的变化。我们发现，在含有SM和16:0、18:1磷脂的简单膜混合物中，OL中总是存在更多的CHL，但长链SM比短链SM从IL中消耗更多的CHL。在更复杂的不对称膜中，长链和短链SM之间的差异被抵消，其中外侧小叶中有更多的饱和尾部。我们提出，长链SM插入相对的IL中会将细胞质CHL推向OL，但外侧小叶中更高的链饱和度补偿了SM链长度的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6615/9034350/c7fd17d95b86/d1ra01464a-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6615/9034350/d9fceb732bde/d1ra01464a-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6615/9034350/05d2cd8c8be2/d1ra01464a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6615/9034350/a7b530572001/d1ra01464a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6615/9034350/c7fd17d95b86/d1ra01464a-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6615/9034350/d9fceb732bde/d1ra01464a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6615/9034350/9a2d9f1e1e3b/d1ra01464a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6615/9034350/05d2cd8c8be2/d1ra01464a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6615/9034350/a7b530572001/d1ra01464a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6615/9034350/c7fd17d95b86/d1ra01464a-f5.jpg

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本文引用的文献

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2
Simulations of Asymmetric Membranes Illustrate Cooperative Leaflet Coupling and Lipid Adaptability.不对称膜的模拟展示了协同的小叶耦合和脂质适应性。
Front Cell Dev Biol. 2020 Jul 21;8:575. doi: 10.3389/fcell.2020.00575. eCollection 2020.
3
Plasma membranes are asymmetric in lipid unsaturation, packing and protein shape.
诱导膜不对称性。
Biophys J. 2023 Jun 6;122(11):2092-2098. doi: 10.1016/j.bpj.2022.12.004. Epub 2022 Dec 6.
质膜在脂类不饱和程度、脂类双层的堆积和膜蛋白形状上都是不对称的。
Nat Chem Biol. 2020 Jun;16(6):644-652. doi: 10.1038/s41589-020-0529-6. Epub 2020 May 4.
4
Direct and indirect cholesterol effects on membrane proteins with special focus on potassium channels.直接和间接的胆固醇对膜蛋白的影响，特别关注钾通道。
Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Aug;1865(8):158706. doi: 10.1016/j.bbalip.2020.158706. Epub 2020 Apr 1.
5
Spontaneous Curvature, Differential Stress, and Bending Modulus of Asymmetric Lipid Membranes.不对称脂质膜的自发曲率、差应力和弯曲弹性系数。
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6
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7
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