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用于增值化学品和食品成分生物制造的下一代原料的微生物利用

Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients.

作者信息

Zhang Congqiang, Ottenheim Christoph, Weingarten Melanie, Ji LiangHui

机构信息

Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (ASTAR), Singapore, Singapore.

Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.

出版信息

Front Bioeng Biotechnol. 2022 Apr 11;10:874612. doi: 10.3389/fbioe.2022.874612. eCollection 2022.

DOI:10.3389/fbioe.2022.874612
PMID:35480982
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9035589/
Abstract

Global shift to sustainability has driven the exploration of alternative feedstocks beyond sugars for biomanufacturing. Recently, C1 (CO, CO, methane, formate and methanol) and C2 (acetate and ethanol) substrates are drawing great attention due to their natural abundance and low production cost. The advances in metabolic engineering, synthetic biology and industrial process design have greatly enhanced the efficiency that microbes use these next-generation feedstocks. The metabolic pathways to use C1 and C2 feedstocks have been introduced or enhanced into industrial workhorses, such as and yeasts, by genetic rewiring and laboratory evolution strategies. Furthermore, microbes are engineered to convert these low-cost feedstocks to various high-value products, ranging from food ingredients to chemicals. This review highlights the recent development in metabolic engineering, the challenges in strain engineering and bioprocess design, and the perspectives of microbial utilization of C1 and C2 feedstocks for the biomanufacturing of value-added products.

摘要

全球向可持续发展的转变推动了生物制造领域对糖类以外替代原料的探索。最近，C1（一氧化碳、二氧化碳、甲烷、甲酸盐和甲醇）和C2（乙酸盐和乙醇）底物因其天然丰富性和低生产成本而备受关注。代谢工程、合成生物学和工业过程设计的进展极大地提高了微生物利用这些下一代原料的效率。通过基因改造和实验室进化策略，利用C1和C2原料的代谢途径已被引入或增强到工业主力菌株中，如大肠杆菌和酵母。此外，微生物经过工程改造后可将这些低成本原料转化为各种高价值产品，从食品成分到化学品。本综述重点介绍了代谢工程的最新进展、菌株工程和生物过程设计中的挑战，以及利用C1和C2原料进行微生物制造增值产品的前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f6a/9035589/949d219beb14/fbioe-10-874612-g010.jpg
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