Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Baima Future Foods Research Institute, Nanjing 211225, China.
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China.
Bioresour Technol. 2023 Jan;367:128259. doi: 10.1016/j.biortech.2022.128259. Epub 2022 Nov 5.
One-carbon (C1) compounds have emerged as a key research focus due to the growth of metabolic engineering and synthetic biology as affordable and sustainable nonfood sugar feedstocks for energy-efficient and environmentally friendly biomanufacturing. This paper summarizes and discusses current developments in C1 compounds for biomanufacturing. First, two primary groups of microbes that use C1 compounds (native and synthetic) are introduced, and the traits, categorization, and functions of C1 microbes are summarized. Second, engineering strategies for C1 utilization are compiled and reviewed, including reconstruction of C1-utilization pathway, enzyme engineering, cofactor engineering, genome-scale modeling, and adaptive laboratory evolution. Third, a review of C1 compounds' uses in the synthesis of biofuels and high-value compounds is presented. Finally, potential obstacles to C1-based biomanufacturing are highlighted along with future research initiatives.
一碳（C1）化合物已成为研究重点，因为代谢工程和合成生物学作为经济实惠且可持续的非食用糖原料，用于能源高效和环境友好的生物制造。本文总结和讨论了用于生物制造的 C1 化合物的最新进展。首先，介绍了使用 C1 化合物（天然和合成）的两种主要微生物群，并总结了 C1 微生物的特性、分类和功能。其次，编译和综述了 C1 利用的工程策略，包括 C1 利用途径的重建、酶工程、辅因子工程、基因组规模建模和适应性实验室进化。第三，综述了 C1 化合物在生物燃料和高价值化合物合成中的应用。最后，强调了基于 C1 的生物制造的潜在障碍，并提出了未来的研究计划。
