Li Shuming, Wang Ke, Hao Shasha, Dang Fuquan, Zhang Zhi-Qi, Zhang Jing
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710119, People's Republic of China.
Anal Chem. 2022 May 10;94(18):6754-6759. doi: 10.1021/acs.analchem.2c00246. Epub 2022 Apr 28.
Large amounts of coexisting contamination in complex biofluid samples impede the quantified veracity of biomarkers, which is the key problem for disease confirmation. Herein, amyloid-like transformed bovine serum albumin inlaid with gold nanoparticles was used as a coating (BGC) on a substrate composed of silicon nanowires (SW; BGC-SW) under ambient conditions. After modification with the recognition group, BGC-SW could serve as an outstanding platform for the selective separation and sensitive detection of biomarkers in complicated biosamples. First, the BGC on SW with a large surface area exhibits excellent adhesion resistance. The attached amounts of contaminations in biofluids were decreased by over 78% compared with native bovine serum albumin (BSA) as the blocking agent. This is because the phase-transformed BSA coating provides stronger interactions with the SW than bare BSA, which results in a tighter attachment and more uniform coverage of the BGC. Furthermore, the gold matrix laid inside the antiadhesive coating ensures simple cross-linking with the recognition groups to selectively capture various biomarkers in complex biofluids and create a gentle release method. Circulating tumor cells (CTCs) were chosen as template biomarkers to verify the application of A-BGC-SW (BGC-SW modified with sgc8-aptamer) in various separation processes of untreated biofluids. The results showed that approximately six cells could be captured from a 1 mL fresh blood sample containing only 10 CTCs. The easy fabrication and excellent antiadhesion property endow A-BGC-SW with great potential in the field of biological separation.
复杂生物流体样本中大量共存的污染物会妨碍生物标志物定量的准确性，这是疾病确诊的关键问题。在此，在环境条件下，将镶嵌有金纳米颗粒的类淀粉样转化牛血清白蛋白用作由硅纳米线（SW）组成的基底上的涂层（BGC），即BGC-SW。在用识别基团修饰后，BGC-SW可作为一个出色的平台，用于复杂生物样本中生物标志物的选择性分离和灵敏检测。首先，具有大表面积的SW上的BGC表现出优异的抗粘附性。与作为封闭剂的天然牛血清白蛋白（BSA）相比，生物流体中污染物的附着量减少了78%以上。这是因为相转化的BSA涂层与SW的相互作用比裸露的BSA更强，这导致BGC的附着更紧密且覆盖更均匀。此外，置于抗粘附涂层内的金基质确保与识别基团进行简单交联，以选择性捕获复杂生物流体中的各种生物标志物，并创建一种温和的释放方法。选择循环肿瘤细胞（CTC）作为模板生物标志物，以验证A-BGC-SW（用sgc8适配体修饰的BGC-SW）在未处理生物流体的各种分离过程中的应用。结果表明，从仅含有10个CTC的1 mL新鲜血液样本中可捕获约6个细胞。易于制备和出色的抗粘附性能赋予A-BGC-SW在生物分离领域巨大的潜力。
