Bioprocess Engineering Laboratory, Centre for Bioenergy, School of Chemical and Biotechnology, SASTRA Deemed To Be University, Thanjavur, 613401, Tamil Nadu, India.
Appl Biochem Biotechnol. 2022 Aug;194(8):3645-3667. doi: 10.1007/s12010-022-03896-7. Epub 2022 Apr 28.
The objective of the work is to examine the potential utilization of Palmyra palm jaggery (PPJ) for the enhancement of bacterial cellulose (BC) production by Gluconacetobacter liquefaciens. To evaluate the culturing condition, the production of BC fermentation was carried out in batch mode using different carbon sources namely glucose, sucrose and PPJ. PPJ in the HS medium (PHS medium) resulted maximum concentration of BC (14.35 ± 0.18 g/L) under shaking condition than other carbon sources in HS medium. The influence of different medium variables including initial pH and nitrogen sources on BC production was investigated using PHS medium under shaking condition. The maximum BC concentration of 17.79 ± 2.4 g/L was obtained in shaking condition at an initial pH of 5.6 using yeast extract as nitrogen source. Stoichiometric equation for the cell growth and BC synthesis was developed using elemental balance approach. The metabolic heat of reaction (40 kcal generated per liter of medium) was evaluated using electron balance approach. Based on the process economic analysis and the yield of BC during the fermentation, PHS medium without nitrogen source could be a promising cost-effective nutrient than HS medium. Thermal stability, crystallinity index and structural characterizations of produced BC using PPJ medium were evaluated using TGA, XRD and FTIR and the obtained results were compared with HS medium containing glucose and sucrose.
这项工作的目的是研究利用帕尔米拉棕榈糖蜜 (PPJ) 来提高液化醋酸杆菌生产细菌纤维素 (BC) 的潜力。为了评估培养条件，使用不同的碳源（葡萄糖、蔗糖和 PPJ）在分批模式下进行 BC 发酵生产。在摇瓶条件下，PPJ 在 HS 培养基（PHS 培养基）中产生的 BC 浓度最高（14.35 ± 0.18 g/L），高于 HS 培养基中其他碳源。在摇瓶条件下，使用 PHS 培养基研究了不同培养基变量（初始 pH 和氮源）对 BC 生产的影响。在初始 pH 为 5.6、使用酵母提取物作为氮源的摇瓶条件下，BC 的最大浓度为 17.79 ± 2.4 g/L。使用元素平衡法开发了细胞生长和 BC 合成的计量方程。使用电子平衡法评估了反应的代谢热（每升培养基产生 40 千卡）。根据工艺经济分析和发酵过程中 BC 的产率，不含氮源的 PHS 培养基可能比含有葡萄糖和蔗糖的 HS 培养基更具成本效益。使用 PPJ 培养基生产的 BC 的热稳定性、结晶度指数和结构特性使用 TGA、XRD 和 FTIR 进行了评估，并将得到的结果与含有葡萄糖和蔗糖的 HS 培养基进行了比较。
