Paranhos Aline Gomes de Oliveira, Pereira Andressa Rezende, da Fonseca Yasmim Arantes, de Queiroz Silva Silvana, de Aquino Sérgio Francisco
Programa de Pós Graduação em Engenharia Ambiental, Escola de Minas, Universidade Federal de Ouro Preto, Campus universitário Morro do Cruzeiro, s/n, Ouro Preto, MG, Brazil.
Departamento de Ciências Biológicas, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Campus universitário Morro do Cruzeiro, s/n, Ouro Preto, MG, Brazil.
Biodegradation. 2022 Jun;33(3):283-300. doi: 10.1007/s10532-022-09980-3. Epub 2022 Apr 28.
Tylosin eliminated in animal waste, during therapeutic treatment, can be efficiently removed in anaerobic systems. The present study investigated the influence of tylosin concentration and assessed its degradation kinetics and the microorganisms involved in each stage of its anaerobic digestion (hydrolysis/acidogenesis; acetogenesis; methanogenesis). The results showed a stimulating effect on methane production with increasing tylosin concentration in the poultry litter up to 80 mg kg tylosin (232.9 NL CH kg SV). As for tylosin degradation, greater removal of antibiotics was observed in the methanogenic phase (88%), followed by acetogenic (84%) and hydrolytic/acidogenic (76%) phases. The higher rate of tylosin degradation obtained in the methanogenic step, is mainly related to the co-metabolic effect exerted by the presence of acetate and its degradation by acetoclastic methanogens. Indeed, metagenomic analyses suggested a syntrophic action between archaea of the genus Methanobacterium, and bacteria such as Clostridium and Flexilinea, which seemed decisive for tylosin degradation.
在治疗过程中，动物粪便中排出的泰乐菌素在厌氧系统中能够被有效去除。本研究调查了泰乐菌素浓度的影响，并评估了其降解动力学以及厌氧消化各阶段（水解/产酸；产乙酸；产甲烷）所涉及的微生物。结果表明，在家禽垫料中，随着泰乐菌素浓度增加至80 mg/kg泰乐菌素（232.9 NL CH/kg SV），对甲烷产生有刺激作用。至于泰乐菌素的降解，在产甲烷阶段观察到抗生素去除率更高（88%），其次是产乙酸阶段（84%）和水解/产酸阶段（76%）。在产甲烷步骤中获得的较高泰乐菌素降解率，主要与乙酸盐的存在所产生的共代谢作用及其被乙酸裂解产甲烷菌降解有关。事实上，宏基因组分析表明，甲烷杆菌属古菌与梭菌属和柔线菌属等细菌之间存在互营作用，这似乎对泰乐菌素降解起决定性作用。
