小鼠卵子精子受体的性质。

Nature of the mouse egg's receptor for sperm.

作者信息

Wassarman P M, Bleil J D, Florman H M, Greve J M, Roller R J, Salzmann G S

出版信息

Adv Exp Med Biol. 1986;207:55-77. doi: 10.1007/978-1-4613-2255-9_5.

Abstract

The zona pellucida is an extracellular coat that surrounds all mammalian eggs. Sperm must penetrate the zona pellucida in order to reach and fuse with the plasma membrane of unfertilized eggs. Penetration is accomplished by a sequence of events involving both egg and sperm. First, sperm must bind to the outer margin of the zona pellucida. Such binding is mediated in a relatively species-specific manner by "sperm receptors" in the zona pellucida. Second, sperm must undergo the "acrosome reaction", a membrane fusion event, in order to traverse the zona pellucida. Here we review results from our own laboratory which demonstrate that, during the course of sperm-egg interaction in mice, zona pellucida glycoprotein ZP3 serves as both receptor for sperm and inducer of the acrosome reaction. Furthermore, we review evidence from our laboratory indicating that the sperm receptor activity of ZP3 is dependent only on its 0-linked carbohydrate components, whereas acrosome reaction-inducing activity is dependent on the polypeptide portion of ZP3 as well.

摘要

透明带是围绕所有哺乳动物卵子的一层细胞外被膜。精子必须穿透透明带才能到达未受精卵的质膜并与之融合。穿透过程是由一系列涉及卵子和精子的事件完成的。首先，精子必须与透明带的外缘结合。这种结合以相对物种特异性的方式由透明带中的“精子受体”介导。其次，精子必须经历“顶体反应”，这是一种膜融合事件，以便穿过透明带。在此，我们回顾了我们自己实验室的结果，这些结果表明，在小鼠精子与卵子相互作用的过程中，透明带糖蛋白ZP3既是精子的受体，也是顶体反应的诱导剂。此外，我们回顾了我们实验室的证据，表明ZP3的精子受体活性仅取决于其O-连接的碳水化合物成分，而顶体反应诱导活性也取决于ZP3的多肽部分。