National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China.
National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
Ecotoxicol Environ Saf. 2022 Jun 1;237:113553. doi: 10.1016/j.ecoenv.2022.113553. Epub 2022 Apr 25.
Insecticide-based vector control measures play an important role in the prevention and control of insect-borne infectious diseases such as malaria; however, insecticide resistance has become a severe global problem for vector control. To date, the metabolic mechanism by which Anopheles sinensis, the most widely distributed malaria vector in China and Asia, detoxifies insecticides is not clear. In this study, the molecular metabolite changes in both the larval and adult stages of deltamethrin susceptible (DS) and deltamethrin-resistant (DR) An. sinensis mosquitoes were analysed by using liquid chromatography tandem mass spectrometry (LC-MS/MS) after exposure to deltamethrin. There were 127 differential metabolites in larval DR An. sinensis and 168 in adults. Five metabolites (glycerophosphocholine, deoxyguanosine, DL-methionine sulfoxide, D-myo-inositol-3-phosphate and N-acetyl-alpha-D-glucosamine1-phosphate) were downregulated in both DR larvae and adults, and one metabolite (aspartyl-glutamine) was upregulated, and the ratio of down- and up-regulation of these metabolites was 5:1. The differential metabolites between the DS and DR mosquitos were mainly classified into organic oxygen compounds, carboxylic acids and their derivatives, glycerophospholipids and purine nucleotides, and the common pathway enriched in both the larval and adult DR An. sinensis was glycerophospholipid metabolism. The findings of this study provide further mechanistic understanding of insecticide resistance in An. sinensis.
基于杀虫剂的病媒控制措施在预防和控制疟疾等虫媒传染病方面发挥着重要作用；然而，杀虫剂抗性已成为病媒控制的一个严重的全球性问题。迄今为止，中国和亚洲分布最广的疟疾媒介按蚊解毒杀虫剂的代谢机制尚不清楚。在这项研究中，采用液相色谱串联质谱法（LC-MS/MS）分析了经溴氰菊酯处理后敏感（DS）和抗性（DR）按蚊幼虫和成虫的分子代谢物变化。DR 按蚊幼虫中有 127 个差异代谢物，成虫中有 168 个。在 DR 幼虫和成虫中均下调了 5 种代谢物（甘油磷酸胆碱、脱氧鸟苷、DL-甲硫氨酸亚砜、D-肌醇-3-磷酸和 N-乙酰-α-D-葡萄糖胺 1-磷酸），上调了 1 种代谢物（天冬酰基-谷氨酸），这些代谢物下调和上调的比例为 5:1。DS 和 DR 蚊子之间的差异代谢物主要分为有机氧化合物、羧酸及其衍生物、甘油磷脂和嘌呤核苷酸，在 DR 按蚊幼虫和成虫中共同富集的途径是甘油磷脂代谢。本研究结果为按蚊杀虫剂抗性的机制提供了进一步的认识。
