Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus, 22010, Pakistan.
Department of Pharmacy, COMSATS University, Islamabad, Abbottabad Campus, 22010, Pakistan.
Int J Biol Macromol. 2022 Jun 1;209(Pt B):1826-1836. doi: 10.1016/j.ijbiomac.2022.04.156. Epub 2022 Apr 26.
Hydrogel membrane dressings with multifunctional tunable properties encompassing biocompatibility, anti-bacterial, oxygen permeability, and adequate mechanical strength are highly preferred for wound healing. The present study aimed to develop biopolymer-based hydrogel membranes for the controlled release of therapeutic agent at the wound site. Toward this end we developed Cefotaxime sodium (CTX) loaded keratin (KR)-pullulan (PL) based hydrogel membrane dressings. All membranes show optimized vapor transmission rate (≥1000 g/ m/day), oxygen permeability >8.2 mg/mL, MTT confirmed good biocompatibility and sufficient tensile strength (17.53 ± 1.9) for being used as a wound dressing. Nonetheless, KR-PL-PVA membranes show controlled CTX release due to enriched hydrophilic moieties which protect the wound from getting infected. In vivo results depict that CTX-KR-PL-PVA membrane group shows a rapid wound closure rate (p < 0.05) with appreciable angiogenesis, accelerated re-epithelization, and excessive collagen deposition at the wound site. These results endorsed that CTX-KR-PL-PVA hydrogel membranes are potential candidates for being used as dressing material in the diabetic wound.
具有多功能可调特性的水凝胶膜，包括生物相容性、抗菌、氧气透过性和足够的机械强度，非常适合伤口愈合。本研究旨在开发基于生物聚合物的水凝胶膜，以在伤口部位控制治疗剂的释放。为此，我们开发了载头孢噻肟钠（CTX）的角蛋白（KR）-普鲁兰（PL）基水凝胶膜敷料。所有的膜都显示出优化的蒸汽透过率（≥1000 g/m/天）、氧气透过率>8.2 mg/mL，MTT 证实了良好的生物相容性和足够的拉伸强度（17.53±1.9），可作为伤口敷料使用。然而，KR-PL-PVA 膜由于富含亲水基团，可保护伤口免受感染，从而显示出 CTX 的控制释放。体内结果表明，CTX-KR-PL-PVA 膜组具有快速的伤口闭合率（p<0.05），并伴有明显的血管生成、加速的再上皮化和过量的胶原蛋白在伤口部位沉积。这些结果表明，CTX-KR-PL-PVA 水凝胶膜是作为糖尿病伤口敷料材料的潜在候选物。
