基于智能手机的量子点侧向流动免疫层析条的研制及其用于抗 SARS-CoV-2 IgG 和中和抗体的超灵敏检测
Development of a smartphone-based quantum dot lateral flow immunoassay strip for ultrasensitive detection of anti-SARS-CoV-2 IgG and neutralizing antibodies.
机构信息
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China; Shenzhen Key Laboratory of Molecular Epidemiology, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China; Guangzhou Blood Center, Guangzhou, China.
出版信息
Int J Infect Dis. 2022 Aug;121:58-65. doi: 10.1016/j.ijid.2022.04.042. Epub 2022 Apr 26.
BACKGROUND
As several vaccines for SARS-CoV-2 have been developed, a large proportion of individuals have been vaccinated worldwide so far. The rapid and accurate immunoassays are urgently needed for detecting the specific virus-neutralizing antibody (NAb), which reflect the protective effect of the vaccines among different populations.
METHODS
In this study, we designed a quantum dot lateral flow immunoassay strip (QD-LFIA) for smartphones for the detection of specific IgG or neutralizing antibodies in SARS-CoV-2 in human serum or whole blood samples. The recombinant receptor binding domain of the SARS-CoV-2 spike protein was used as the antigen to combine with NAb or angiotensin-converting enzyme 2.
RESULTS
Among 81 patients who recovered from COVID-19 who were diagnosed using the nucleic acid test initially, 98.8% (80/81) were positive for IgG and 88.9% (72/81) were positive for NAb by QD-LFIA. Among 64 individuals inoculated with inactivated vaccines and six subunit vaccines, 90% (63/70) were positive for IgG and 82.9% (58/70) were positive for NAb by QD-LFIA, whereas no cross-reaction was found in 150 healthy blood donors, two patients with influenza B, and three patients with common cold.
CONCLUSION
The established platform could achieve a rapid and accurate detection of NAb specific to SARS-CoV-2, which could be used for detecting the protective effect of the vaccines in areas of world that currently affected by the pandemic.
背景
随着几种针对 SARS-CoV-2 的疫苗的开发，迄今为止，全球已有很大一部分人接种了疫苗。目前，迫切需要快速准确的免疫分析方法来检测针对特定病毒的中和抗体 (NAb)，该抗体反映了不同人群中疫苗的保护作用。
方法
在这项研究中，我们设计了一种用于智能手机的量子点侧向流动免疫分析条（QD-LFIA），用于检测人血清或全血样本中 SARS-CoV-2 的特异性 IgG 或中和抗体。SARS-CoV-2 刺突蛋白的重组受体结合域被用作抗原，与 NAb 或血管紧张素转换酶 2 结合。
结果
在最初通过核酸检测诊断为 COVID-19 康复的 81 名患者中，98.8%（80/81）的 IgG 呈阳性，88.9%（72/81）的 NAb 呈阳性，QD-LFIA 检测。在接种灭活疫苗和 6 种亚单位疫苗的 64 名个体和 6 名个体中，90%（63/70）的 IgG 呈阳性，82.9%（58/70）的 NAb 呈阳性，而在 150 名健康献血者、2 名乙型流感患者和 3 名普通感冒患者中未发现交叉反应。
结论
所建立的平台可以快速准确地检测针对 SARS-CoV-2 的 NAb，可用于检测目前受大流行影响地区疫苗的保护作用。
Zotero 插件