Demedts P, Vermeulen-Van Overmeir C, Wery M
Am J Trop Med Hyg. 1987 Mar;36(2):257-63. doi: 10.4269/ajtmh.1987.36.257.
An enzyme-linked immunosorbent assay for malaria, based upon duplicate testing of serum samples on both a crude Plasmodium falciparum antigen and a red blood cell control antigen, is evaluated. Results were analyzed using the Student's t-test for identification of positive serum samples (t greater than or equal to 2.92, P less than or equal to 0.05) and for calculation of the mean difference in absorbance values (delta ABS) obtained between the P. falciparum wells and the control wells. Cross-evaluation with the IFA test for P. falciparum antibodies gave 89.6% concording positive or negative results. Among discrepant sera 8.35% were ELISA+/IFA- and 2.05% ELISA-/IFA+. In addition, delta ABS values in ELISA were highly correlated to titers obtained in immunofluorescence (r = 0.80, P less than 0.001). The results confirm the high degree of species-specificity of the ELISA using P. falciparum crude antigen. The necessity of the simultaneous use of red blood cell control antigen with a crude plasmodial antigen is demonstrated by comparing the presented results with those obtained on the P. falciparum antigen only.
评估了一种基于对血清样本在恶性疟原虫粗抗原和红细胞对照抗原上进行重复检测的疟疾酶联免疫吸附测定法。使用学生t检验分析结果，以鉴定阳性血清样本（t大于或等于2.92，P小于或等于0.05），并计算恶性疟原虫孔和对照孔之间获得的吸光度值的平均差异（δABS）。与恶性疟原虫抗体的间接免疫荧光试验进行交叉评估，结果显示89.6%的阳性或阴性结果一致。在结果不一致的血清中，8.35%为酶联免疫吸附测定法阳性/间接免疫荧光试验阴性，2.05%为酶联免疫吸附测定法阴性/间接免疫荧光试验阳性。此外，酶联免疫吸附测定法中的δABS值与免疫荧光中获得的滴度高度相关（r = 0.80，P小于0.001）。结果证实了使用恶性疟原虫粗抗原的酶联免疫吸附测定法具有高度的种属特异性。通过将呈现的结果与仅在恶性疟原虫抗原上获得的结果进行比较，证明了同时使用红细胞对照抗原和粗疟原虫抗原的必要性。
