以完整恶性疟原虫为抗原的斑点免疫结合试验（DIBA）在恶性疟免疫诊断中的应用

Dot-immunobinding assay (DIBA) with integral Plasmodium falciparum as antigen in immuno-diagnosis of falciparum malaria.

作者信息

Wang J, Zhang L X, Meng H, Zhang Y M, Chen C Y, Feng X P

机构信息

Department of Immunology, Chinese Academy of Preventive Medicine, Shanghai.

出版信息

Southeast Asian J Trop Med Public Health. 1989 Dec;20(4):523-8.

PMID:2700844

Abstract

Among the available immuno-diagnostic methods of parasitoses, dot-immunobinding assay (DIBA) has been proved to be promising for its high sensitivity and specificity, easy performance, lack of need of special equipment, and consequently its practical usage in field work. In previously reported tests, soluble antigen was used, thus a sonicator and an ultracentrifuge were required to produce the antigen. This paper reports the application of integral P. falciparum as antigen in DIBA to detect antibodies in falciparum malaria cases. Of 52 sera from falciparum malaria patients tested, 49 (94.2%) showed positive reactions, which was similar to the result using soluble antigen in DIBA (96.2%) and was higher than that in IFA (86.5%) and ELISA (80.8%). No false positive was revealed in 48 control sera from healthy individuals and sera from visceral leishmaniasis, paragonimiasis, fasciolopsiasis and schistosomiasis patients.

摘要

在现有的寄生虫病免疫诊断方法中，斑点免疫结合试验（DIBA）因其高灵敏度、高特异性、操作简便、无需特殊设备，因而在现场工作中具有实际应用价值，已被证明是一种很有前景的方法。在先前报道的试验中，使用的是可溶性抗原，因此需要用超声处理器和超速离心机来制备抗原。本文报道了将恶性疟原虫完整虫体作为抗原应用于DIBA中，以检测恶性疟病例中的抗体。在检测的52份恶性疟患者血清中，49份（94.2%）呈阳性反应，这与在DIBA中使用可溶性抗原的结果（96.2%）相似，且高于间接荧光抗体试验（IFA）（86.5%）和酶联免疫吸附测定（ELISA）（80.8%）的结果。在48份来自健康个体的对照血清以及来自内脏利什曼病、肺吸虫病、姜片吸虫病和血吸虫病患者的血清中未发现假阳性。