Circulating bile acid concentrations and non-alcoholic fatty liver disease in Guatemala.

BACKGROUND

Non-alcoholic fatty liver disease (NAFLD) is a major liver disease worldwide. Bile acid dysregulation may be a key feature in its pathogenesis and progression.

AIMS

To characterise the relationship between bile acid levels and NAFLD at the population level METHODS: We conducted a cross-sectional study in Guatemala in 2016 to examine the prevalence of NAFLD. Participants (n = 415) completed questionnaires, donated blood samples and had a brief medical exam. NAFLD was determined by calculation of the fatty liver index. The levels of 15 circulating bile acids were determined by LC-MS/MS. Adjusted prevalence odds ratios (POR ) and 95% CI were calculated to examine the relationships between bile acid levels (in tertiles) and NAFLD.

RESULTS

Persons with NAFLD had significantly higher levels of the conjugated primary bile acids glycocholic acid (GCA) (POR  = 1.85), taurocholic acid (TCA) (POR  = 2.45) and taurochenodeoxycholic acid (TCDCA) (POR  = 2.10), as well as significantly higher levels the unconjugated secondary bile acid, deoxycholic acid (DCA) (POR  = 1.78) and its conjugated form, taurodeoxycholic acid (TDCA) (POR  = 1.81).

CONCLUSIONS

The bile acid levels of persons with and without NAFLD differed significantly. Among persons with NAFLD, higher levels of the conjugated forms of CA (i.e. GCA, TCA) and the secondary bile acids that derive from CA (i.e. DCA, TDCA) may indicate there is hepatic overproduction of CA, which may affect the liver via aberrant signalling mediated by the bile acids.