Yan Yan, Wang Shiqing, Gu Jinping, Min Zhihong, Wang Ruizhi
Department of Obstetrics, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, PR China.
Department of Radiology, Huadong Hospital, Fudan University, Shanghai 200040, PR China.
J Biomed Nanotechnol. 2022 Feb 1;18(2):474-480. doi: 10.1166/jbn.2022.3262.
Diabetes is a widespread metabolic syndrome, an important complication during pregnancy. Most cases are type 2 diabetes, which has attracted the attention of the World Health Organization. The typical feature of T2DM is insulin resistance (IR). Its mechanism remains unclear, but it mainly manifests through parameters like insulin sensitivity, blood glucose level, and liver stability. Oxidative stress and insulin transduction play an important role in IR. This study simulates the disease situation, establishes a high-fat and high-fructose-induced model induced by tert-butyl hydroperoxide (tBHP), and adopts nano-sized oleanolic acid combined with lipid-lowering ketones to explore improvement in the IR mechanism. We found combining nano-sized oleanolic acid and lipid-lowering ketones can slow down the weight gain process in rats, reduce fasting blood glucose levels, increase the insulin sensitivity index, reduce the serum MDA, NO, and triglyceride content, and increase SOD, CAT activity. In summary, our results show that the combined use of nano-sized oleanolic acid and lipid-lowering ketone in pregnant rats with double height can reduce glucose metabolism, delay lipid production, and reduce oxidative stress, which is useful for further treatment and interpretation of T2DM The mechanism provides a theoretical basis.
糖尿病是一种广泛存在的代谢综合征，是孕期的一种重要并发症。大多数病例为2型糖尿病，已引起世界卫生组织的关注。2型糖尿病的典型特征是胰岛素抵抗（IR）。其机制尚不清楚，但主要通过胰岛素敏感性、血糖水平和肝脏稳定性等参数表现出来。氧化应激和胰岛素转导在胰岛素抵抗中起重要作用。本研究模拟疾病情况，建立叔丁基过氧化氢（tBHP）诱导的高脂高果糖模型，并采用纳米齐墩果酸联合降脂酮来探索胰岛素抵抗机制的改善情况。我们发现纳米齐墩果酸与降脂酮联合使用可减缓大鼠体重增加过程，降低空腹血糖水平，提高胰岛素敏感性指数，降低血清丙二醛、一氧化氮和甘油三酯含量，并提高超氧化物歧化酶、过氧化氢酶活性。综上所述，我们的结果表明，纳米齐墩果酸与降脂酮联合应用于双高妊娠大鼠可降低糖代谢，延缓脂质生成，减轻氧化应激，为进一步治疗和阐释2型糖尿病的机制提供了理论依据。
