Delarue J C, Mouriesse H, Contesso G, Bonnay M, May-Levin F, Sancho-Garnier H, Travagli J P, Lacombe M J
Ann Biol Clin (Paris). 1986;44(6):629-33.
The dosage of estrogenic receptors (ER) was performed on 80 cytosols of human breast carcinomas "immediately operable", by using a methode consisting of a single saturating dose (5 nM) with carbon-dextran and a new immuno-enzymatic method commercialized by ABBOTT laboratories using anti-ER monoclonal antibodies. The concordance is excellent between the two methods in terms of positivity. The results are significantly higher with the ER-IEA method than with the biochemical method (p. less than 1.10(-5]. The regression of the ER-IEA method as compared to the biochemical method is linear with a regression line: Y (ER-IEA) = 0.82 X (ER-Biochem) + 0.76 (logo10 conc. fmoles/g tissue). The correlation is good between the two methods (r = 0.90). A significant relationship (p less than 0.01) has also been observed with the histological grade of the carcinoma. Finally, the immuno-enzymatic technique seems particularly well adapted to small biopsies and slightly positive tumors.
采用由单次饱和剂量（5 nM）的碳葡聚糖以及雅培实验室商业化的使用抗雌激素受体（ER）单克隆抗体的新型免疫酶法，对80例“可立即手术”的人乳腺癌细胞溶质进行了雌激素受体剂量检测。在阳性方面，两种方法的一致性极佳。雌激素受体免疫酶分析法（ER-IEA）的结果显著高于生化方法（p＜1.10⁻⁵）。与生化方法相比，ER-IEA方法的回归呈线性，回归线为：Y（ER-IEA） = 0.82X（ER-生化法） + 0.76（log₁₀浓度，飞摩尔/克组织）。两种方法之间的相关性良好（r = 0.90）。还观察到与癌组织学分级存在显著关系（p＜0.01）。最后，免疫酶技术似乎特别适用于小活检标本和弱阳性肿瘤。
