Spillert C R, Lazaro E J
Am Surg. 1987 Mar;53(3):164-6.
Isolated human monocytes generate tissue factor when stimulated with endotoxins. This tissue factor generation provides a convenient marker of monocyte activation. Furthermore, the only circulating blood cell capable of generating large quantities of tissue factor is the monocyte. Therefore, the addition of endotoxin to citrated blood, and the determination of the recalcification times after incubation, yields a measure of monocyte activation. In order to determine whether monocyte activation as measured by this technique varies between patients with carcinoma and healthy volunteers, recalcification times were evaluated. The recalcification time and range for 19 healthy volunteers (controls) was 6.55 min (5.3-8.5) for the saline incubated sample and 5.69 min (4.6-7.2) for the endotoxin-activated sample. The results for 13 patients with carcinoma are 4.81 min (3.3-6.3) for the former and 3.17 min (2.0-4.3) for the latter. These results show that the longest recalcification time with endotoxin incubation for patients with carcinoma was lower than the lowest recalcification time in the control group. Whether this simple test can be of use in the diagnosis and monitoring of patients with carcinomas is currently being investigated.
分离出的人类单核细胞在内毒素刺激下会产生组织因子。这种组织因子的产生为单核细胞活化提供了一个便利的标志物。此外，唯一能够产生大量组织因子的循环血细胞是单核细胞。因此，向枸橼酸盐抗凝血液中添加内毒素，并在孵育后测定再钙化时间，可得出单核细胞活化程度的一个指标。为了确定通过该技术测定的单核细胞活化在癌症患者和健康志愿者之间是否存在差异，对再钙化时间进行了评估。19名健康志愿者（对照组）的生理盐水孵育样本的再钙化时间及范围为6.55分钟（5.3 - 8.5），内毒素激活样本的再钙化时间及范围为5.69分钟（4.6 - 7.2）。13名癌症患者的相应结果是，前者为4.81分钟（3.3 - 6.3），后者为3.17分钟（2.0 - 4.3）。这些结果表明，癌症患者内毒素孵育后的最长再钙化时间低于对照组的最短再钙化时间。目前正在研究这种简单检测方法是否可用于癌症患者的诊断和监测。
