Department of Pediatrics and Adolescent Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
Core Facility of Clinical Chemistry, University Medical Centre Ulm, Ulm, Germany.
Int J Lab Hematol. 2022 Aug;44(4):729-737. doi: 10.1111/ijlh.13846. Epub 2022 Apr 29.
Capillary sampling of blood counts is a well-established alternative to venipuncture in paediatrics. However, the sampling method has to be considered when interpreting test results, as measurements differ. Ethical and practical considerations prevent simultaneous venous and capillary sample acquisition in comprehensive paediatric cohorts that span all ages for the purpose of a direct method comparison, resulting in uncertainty regarding the interpretation of capillary test results.
We applied a data mining method to calculate the differences between capillary and venous blood count analytes using laboratory data collected during patient care. We examined 486 401 blood counts performed between 2010 and 2017 in two German paediatric tertiary care centers in children from birth to 18 years analysed on SYSMEX XE-2100 and SYSMEX XE-5000 devices, and analysed the differences between capillary and venous test results in 15 218 paired samples performed within 24 h.
We identified the mean systematic differences between capillary and venous (capillary-venous) test results for haemoglobin (+6.5 g/L), haematocrit (+2.38%), platelet count (-7.01 × 10 /l), red cell count (+0.18 × 10 /L), white cell count (-0.64 × 10 /L), mean corpuscular cell volume (+2.07 fl), mean corpuscular haemoglobin (+0.33 pg), mean corpuscular haemoglobin concentration (-4.4 g/L) and red cell distribution width (+0.40%). The effect of age on these mean deltas is negligible, while the levels of test results influence the difference between capillary and venous test results in most analytes.
Our results improve guidance regarding the interpretation of capillary test results for children of all ages and in both physiological and pathological ranges.
毛细血管采血是儿科中一种成熟的静脉采血替代方法。然而，在解释检测结果时必须考虑到采样方法，因为测量结果有所不同。出于伦理和实际考虑，在涵盖所有年龄段的综合儿科队列中，无法同时采集静脉和毛细血管样本以进行直接方法比较，这导致对于毛细血管检测结果的解释存在不确定性。
我们应用数据挖掘方法，使用患者护理过程中收集的实验室数据来计算毛细血管和静脉血细胞分析物之间的差异。我们检查了 2010 年至 2017 年期间在德国两家儿科三级保健中心进行的 486401 次血液计数，这些血液计数是在 SYSMEX XE-2100 和 SYSMEX XE-5000 仪器上对出生至 18 岁的儿童进行的，并且分析了在 24 小时内进行的 15218 对毛细血管和静脉检测结果之间的差异。
我们确定了血红蛋白（+6.5 g/L）、血细胞比容（+2.38%）、血小板计数（-7.01×10 / l）、红细胞计数（+0.18×10 / L）、白细胞计数（-0.64×10 / L）、平均红细胞体积（+2.07 fl）、平均红细胞血红蛋白（+0.33 pg）、平均红细胞血红蛋白浓度（-4.4 g/L）和红细胞分布宽度（+0.40%）之间的毛细血管-静脉测试结果的平均系统差异。年龄对这些平均差值的影响可以忽略不计，而检测结果的水平在大多数分析物中影响毛细血管和静脉检测结果之间的差异。
我们的研究结果改进了对所有年龄段儿童的毛细血管检测结果的解释，涵盖了生理和病理范围。
