Department of Chemistry, School of Physical Sciences, University of California Irvine, Irvine, California 92697, United States.
Department of Chemical and Biomolecular Engineering, Henry Samueli School of Engineering, University of California Irvine, Irvine, California 92697, United States.
ACS Appl Mater Interfaces. 2022 May 11;14(18):20729-20738. doi: 10.1021/acsami.2c03111. Epub 2022 Apr 29.
Herein, we report the first example of programmable living materials constructed with a dynamic covalent interface between designed synthetic polymers and engineered cells. We identified a molecular motif that forms reversible dynamic covalent bonds on the cell surface. Combining block copolymers bearing this motif with genetically engineered yields programmable living materials that can be equipped with functionalities such as biosensing and on-demand elution of recombinant proteins. Encapsulated cells in these living materials could be reversibly retrieved and subjected to biological analyses. Further, the block copolymer in these living materials could be recycled to produce a new batch of living materials. This work advances the current capabilities in engineered living materials, establishes the groundwork for building a myriad of synthetic polymeric materials integrating engineered living cells, and provides a platform for understanding the biology of cells confined within materials.
在此，我们报告了首例使用设计合成聚合物与工程细胞之间的动态共价界面构建的可编程活体材料。我们鉴定出一种分子模体，它可在细胞表面形成可逆的动态共价键。将带有这种结构单元的嵌段共聚物与基因工程细胞结合，得到的可编程活体材料可具有生物传感和按需洗脱重组蛋白等功能。这些活体材料中包封的细胞可以被可逆地回收并进行生物学分析。此外，这些活体材料中的嵌段共聚物可以回收，以生产新一批活体材料。这项工作推进了工程活体材料的现有能力，为构建无数种集成了工程活细胞的合成聚合物材料奠定了基础，并为理解受限在材料中的细胞生物学提供了一个平台。
