Department of Neurology, Henan University People's Hospital, Zhengzhou, China.
Department of Neurology, Henan Provincial People's Hospital, Zhengzhou, China.
Neurol Sci. 2022 Aug;43(8):4753-4759. doi: 10.1007/s10072-022-06093-w. Epub 2022 Apr 29.
Arylsulfatase A (ARSA), a lysosomal enzyme, has been shown to inhibit the aggregation and propagation of α-synuclein (α-syn) through its molecular chaperone function. The relationship between ARSA levels and Parkinson's disease (PD) in the Chinese Han population remains controversial, and few quantitative research studies have investigated the relationship between plasma ARSA levels and PD.
The purpose of this study was to investigate the relationships between ARSA levels and cognitive function in PD patients and to evaluate the association of ARSA and α-syn levels with nonmotor symptoms.
Enzyme-linked immunosorbent assay (ELISA) was used to measure the plasma ARSA and α-syn levels in 50 healthy controls, 120 PD patients (61 PD patients with no cognitive impairment (PD-NCI) and 59 PD patients with cognitive impairment (PD-CI)). Motor symptoms and nonmotor symptoms (cognitive function, Unified Parkinson's Disease Rating Scale (UPDRS) score, depression, anxiety, constipation, olfactory dysfunction, sleep disruption, and other symptoms) were assessed with the relevant scales. The Kruskal-Wallis H test was used for comparison between groups, and Pearson/Spearman analysis was used for correlation analysis.
The plasma ARSA concentrations were lower in the PD-CI group than in the PD-NCI group. The plasma α-syn levels in the PD-CI group were higher than those in the healthy control group, and the plasma ARSA levels were correlated with the Mini-Mental State Examination (MMSE scores) and Hoehn and Yahr (H-Y) stage.
We used a quantitative assessment method to show that low plasma ARSA levels and high α-syn levels are related to cognitive impairment in PD patients. Plasma ARSA levels gradually decrease with PD progression.
芳基硫酸酯酶 A（ARSA）是一种溶酶体酶，其分子伴侣功能已被证明可抑制α-突触核蛋白（α-syn）的聚集和传播。ARSA 水平与中国汉族人群帕金森病（PD）之间的关系仍存在争议，很少有定量研究探讨血浆 ARSA 水平与 PD 之间的关系。
本研究旨在探讨 PD 患者 ARSA 水平与认知功能的关系，并评估 ARSA 和 α-syn 水平与非运动症状的关系。
采用酶联免疫吸附试验（ELISA）检测 50 名健康对照者、120 名 PD 患者（61 名无认知障碍的 PD 患者（PD-NCI）和 59 名有认知障碍的 PD 患者（PD-CI））的血浆 ARSA 和 α-syn 水平。采用相关量表评估运动症状和非运动症状（认知功能、统一帕金森病评定量表（UPDRS）评分、抑郁、焦虑、便秘、嗅觉障碍、睡眠障碍和其他症状）。采用 Kruskal-Wallis H 检验进行组间比较，采用 Pearson/Spearman 分析进行相关性分析。
PD-CI 组的血浆 ARSA 浓度低于 PD-NCI 组。PD-CI 组的血浆 α-syn 水平高于健康对照组，且血浆 ARSA 水平与简易精神状态检查（MMSE 评分）和 Hoehn 和 Yahr（H-Y）分期相关。
我们采用定量评估方法表明，血浆 ARSA 水平降低和 α-syn 水平升高与 PD 患者的认知障碍有关。随着 PD 进展，血浆 ARSA 水平逐渐降低。
