Favreau L V, Schenkman J B
Biochem Biophys Res Commun. 1987 Feb 13;142(3):623-30. doi: 10.1016/0006-291x(87)91460-4.
Cytochrome P-450 dependent hydroxylation of testosterone has been measured in hepatic microsomes of control, diabetic and insulin-treated diabetic rats. The observed decrease in testosterone 16 alpha-hydroxylase activity in diabetes, an activity previously shown to be largely due to RLM5, was accompanied by a dramatic decrease in immunodetectable RLM5. Diabetic rats which received insulin had elevated testosterone 16 alpha-hydroxylase activity relative to the diabetic animals, which was accompanied by a corresponding increase in the levels of RLM5. These results provide evidence that specific constitutive cytochrome P-450 enzymes are altered in the diabetic state and that these changes are not permanent since they can be overcome, at least partially, by insulin replacement therapy.
已在对照、糖尿病和胰岛素治疗的糖尿病大鼠的肝微粒体中测定了细胞色素P-450依赖性睾酮羟化作用。糖尿病状态下观察到的睾酮16α-羟化酶活性降低，该活性先前已证明主要归因于RLM5，同时免疫可检测到的RLM5也显著降低。接受胰岛素治疗的糖尿病大鼠相对于未治疗的糖尿病动物，其睾酮16α-羟化酶活性升高，同时RLM5水平也相应增加。这些结果证明，在糖尿病状态下特定的组成型细胞色素P-450酶发生了改变，并且这些变化不是永久性的，因为至少部分地可以通过胰岛素替代疗法来克服。
