Kimura K, Ogura M
Department of Biochemistry, Tottori University School of Medicine.
Biol Chem Hoppe Seyler. 1988 Oct;369(10):1117-20. doi: 10.1515/bchm3.1988.369.2.1117.
The activity of 12 alpha-hydroxylase in hepatic microsomes from normal, streptozotocin-induced diabetic, and insulin-treated diabetic rats was studied with 7 alpha-hydroxy-4-cholesten-3-one as a substrate. In the diabetic rats, the 12 alpha-hydroxylase activity was about 50% lower than in the normal rats. Treatment of the diabetics with insulin cancelled the reduction of the activity. These results show that an insulin-deficient state causes a paradoxical decrease in the activity of the key enzyme for cholic acid formation.
以7α-羟基-4-胆甾烯-3-酮为底物，研究了正常大鼠、链脲佐菌素诱导的糖尿病大鼠以及胰岛素治疗的糖尿病大鼠肝微粒体中12α-羟化酶的活性。糖尿病大鼠的12α-羟化酶活性比正常大鼠低约50%。用胰岛素治疗糖尿病大鼠可消除酶活性的降低。这些结果表明，胰岛素缺乏状态会导致胆酸形成关键酶的活性出现反常降低。
