Laboratoire National de l'Elevage et de Recherches Vétérinaires (ISRA-LNERV), Institut Sénégalais de Recherches Agricoles, BP: 2057 Dakar-Hann, Sénégal.
Laboratoire National de l'Elevage et de Recherches Vétérinaires (ISRA-LNERV), Institut Sénégalais de Recherches Agricoles, BP: 2057 Dakar-Hann, Sénégal.
Acta Trop. 2022 Aug;232:106487. doi: 10.1016/j.actatropica.2022.106487. Epub 2022 Apr 26.
Bluetongue (BT) is an infectious, arthropod-borne viral disease of domestic and wild ruminants. The disease causes animal mortality, production decrease and commercial limits for herds. Despite the active circulation of the disease in the world, few studies have been carried out in Senegal. The objective of this study was to assess the current prevalence of BT in small ruminants and the serotypes circulating in Senegal. A cross-sectional study was conducted in the fourteen regions of Senegal. After the sampling campaign, sera collected in sheep and goats herds were screened for the presence of Bluetongue virus (BTV) specific antibodies using c-Elisa. The whole blood of seropositive animals was further analyzed by RT-qPCR and positive samples were typed to identify BTV serotypes. Analysis of several risk factors such as age, sex and species of animals was performed using logistic regression. The overall seroprevalence of BTV in Senegal was 72.6% (95% CI: 70.3-74.9%) with 75.9% (95% CI: 72.2-79.5%) in goat and 70.6% (95% CI: 67.5-73.6%) in sheep. Female (prevalence=77.1%) and adult (prevalence=80%) animals showed the highest seropositivity to BTV compared respectively to male (55.7%, p=6.133e-09) and young (49.4%, p < 2.2e-16). The RT-qPCR results showed the presence of BT viral genome in 359 small ruminants. The results obtained from serological and genotyping studies showed an active spread of the Bluetongue virus in domestic ruminants and phylogenetic analysis showed that the BTV-2 is one of the circulating serotypes in Senegal. This study allows having baseline information for controlling Bluetongue in Senegal.
蓝舌病（BT）是一种传染性的、节肢动物传播的病毒性疾病，影响家养和野生反刍动物。该疾病导致动物死亡、生产减少以及畜群的商业限制。尽管该病在世界范围内广泛传播，但在塞内加尔开展的研究却很少。本研究旨在评估 BT 在小反刍动物中的当前流行率以及在塞内加尔流行的血清型。在塞内加尔的 14 个地区进行了一项横断面研究。在采样运动之后，使用 c-Elisa 筛查绵羊和山羊群血清中是否存在蓝舌病病毒（BTV）特异性抗体。对血清阳性动物的全血进一步进行 RT-qPCR 分析，对阳性样本进行分型以鉴定 BTV 血清型。使用逻辑回归分析了年龄、性别和动物种类等几个风险因素。塞内加尔 BTV 的总体血清阳性率为 72.6%（95%CI：70.3-74.9%），其中山羊为 75.9%（95%CI：72.2-79.5%），绵羊为 70.6%（95%CI：67.5-73.6%）。雌性（阳性率=77.1%）和成年（阳性率=80%）动物的 BTV 血清阳性率最高，分别比雄性（阳性率=55.7%，p=6.133e-09）和幼年（阳性率=49.4%，p<2.2e-16）动物高。RT-qPCR 结果显示在 359 只小反刍动物中存在 BT 病毒基因组。血清学和基因分型研究的结果表明，蓝舌病病毒在国内反刍动物中广泛传播，系统发育分析表明 BTV-2 是塞内加尔流行的血清型之一。本研究为塞内加尔的蓝舌病控制提供了基线信息。
