Jagiellonian University Medical College, Faculty of Medicine, Chair of Medical Biochemistry, 7 Kopernika St., 31-034, Kraków, Poland.
Department of Physiology and Experimental Pathophysiology, Laboratory of the Centre for Preclinical Research, Medical University of Warsaw, 1B Banacha St., 02-097, Warsaw, Poland; Veterinary Diagnostic Laboratory ALAB Bioscience, ALAB plus sp. z o.o., 13 Krucza St., 05-090, Rybie, Poland.
Biochimie. 2022 Aug;199:130-138. doi: 10.1016/j.biochi.2022.04.013. Epub 2022 Apr 27.
The prevalence of hypertension increases with age, but the mechanisms linking this phenomenon are not well understood. Hydrogen sulfide (HS) may be involved in this process, as it plays a role in the cardiovascular system, affecting blood pressure and heart and kidney functions. The aim of this study was to evaluate the influence of hypertension and aging on sulfur-containing compounds metabolism in the hearts and kidneys of Wistar Kyoto (WKY) and Spontaneously Hypertensive Rats (SHR) of different age groups. We determined the expression and activity of four enzymes participating in HS production: cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CTH), 3-mercaptopyruvate sulfurtransferase (MPST), and thiosulfate sulfurtransferase (TST). The levels of reduced/oxidized glutathione, cysteine, cystine, and cystathionine, and the ability of tissues to form hydrogen sulfide were also investigated. Tissues obtained from younger WKY rats produced the highest amounts of HS. The effect of hypertension on the metabolism of sulfur-containing compounds was manifested by a decrease in sulfane sulfur concentrations in heart homogenates and a decrease in CTH activity in the kidneys. The hearts and kidneys of older WKY rats were characterized by lower MPST or CTH gene expression, respectively, compared to younger animals. Our study demonstrates that hypertension and aging influence cardiac and renal sulfur-containing compounds metabolism and reduce HS production. Furthermore, we showed that MPST plays a major role in the production of hydrogen sulfide in the heart and CTH in the kidneys of rats.
高血压的患病率随着年龄的增长而增加，但将这种现象联系起来的机制尚不清楚。硫化氢（HS）可能参与了这一过程，因为它在心血管系统中发挥作用，影响血压以及心脏和肾脏功能。本研究旨在评估高血压和衰老对不同年龄组 Wistar Kyoto（WKY）和自发性高血压大鼠（SHR）心脏和肾脏中含硫化合物代谢的影响。我们测定了参与 HS 生成的四种酶的表达和活性：胱硫醚β-合酶（CBS）、胱硫醚γ-裂解酶（CTH）、3-巯基丙酮酸硫转移酶（MPST）和硫代硫酸盐硫转移酶（TST）。还研究了还原/氧化型谷胱甘肽、半胱氨酸、胱氨酸和胱硫醚的水平以及组织形成硫化氢的能力。来自年轻 WKY 大鼠的组织产生了最多量的 HS。高血压对含硫化合物代谢的影响表现为心脏匀浆中磺基硫含量降低以及肾脏中 CTH 活性降低。与年轻动物相比，老年 WKY 大鼠的心脏和肾脏的 MPST 或 CTH 基因表达分别降低。本研究表明，高血压和衰老影响心脏和肾脏含硫化合物代谢并减少 HS 生成。此外，我们表明 MPST 在心脏中产生 HS 以及 CTH 在肾脏中起主要作用。
