Key Laboratory of Food Nutrition and Safety, Ministry of Education-Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, PR China; State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, PR China; College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China.
College of Public Health, Zunyi Medical University, Guizhou 563006, PR China.
Int J Biol Macromol. 2022 Jun 1;209(Pt B):1815-1825. doi: 10.1016/j.ijbiomac.2022.04.154. Epub 2022 Apr 26.
The crude polysaccharide LDP was extracted from mycelia of Lactarius deliciosus Gray and then purified by DEAE-52 cellulose and Sephadex G-200 to obtain a novel polysaccharide named LDP-CP. LDP-CP was mainly composed of mannose, glucose and galactose with an average molecular weight of 2.33 × 10 kDa. The structure of LDP-CP was determined by FT-IR, methylation and NMR analysis, and the results showed that the sugar linkage units of LDP-CP were composed of (1 → 3)-linked β-D-Manp, (1 → 2,4)-linked α-D-Manp, (1→)-linked α-D-Manp, (1 → 4)-linked β-D-Glcp, (1 → 2)-linked β-D-Manp, (1 → 4,6)-linked α-D-Manp, (1 → 4)-linked α-D-Galp, (1 → 2,3)-linked α-D-Glcp and (1→)-linked α-D-Glcf. The protective effects of LDP and LDP-CP on PC12 cells against HO-induced oxidative injury were exhibited by enhancing cell viability and morphological protection. The improvement to the level of LDH, SOD and GSH further indicated that LDP and LDP-CP had ability to alleviate HO-induced oxidative damage on PC12 cells. The polysaccharides in Lactarius deliciosus Gray mycelia exhibited the great advantages in the management of oxidative toxicity, which indicated that the polysaccharides can be further developed in application of natural functional food source.
从美味牛肝菌菌丝体中提取粗多糖 LDP，然后用 DEAE-52 纤维素和 Sephadex G-200 纯化得到一种新型多糖，命名为 LDP-CP。LDP-CP 主要由甘露糖、葡萄糖和半乳糖组成，平均分子量为 2.33×10 kDa。通过 FT-IR、甲基化和 NMR 分析确定了 LDP-CP 的结构，结果表明 LDP-CP 的糖连接单元由 (1→3)-β-D-Manp、(1→2,4)-α-D-Manp、(1→)-α-D-Manp、(1→4)-β-D-Glcp、(1→2)-β-D-Manp、(1→4,6)-α-D-Manp、(1→4)-α-D-Galp、(1→2,3)-α-D-Glcp 和 (1→)-α-D-Glcf 组成。LDP 和 LDP-CP 通过提高细胞活力和形态保护，对 PC12 细胞对抗 HO 诱导的氧化损伤表现出保护作用。LDH、SOD 和 GSH 水平的提高进一步表明，LDP 和 LDP-CP 具有减轻 HO 诱导的 PC12 细胞氧化损伤的能力。美味牛肝菌菌丝体中的多糖在管理氧化毒性方面具有巨大优势，这表明多糖可以进一步开发应用于天然功能性食品来源。
