National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Khlong Luang, 12120, Pathum Thani, Thailand.
Department of Agricultural Technology, Faculty of Science and Technology, Thammasat University, Rangsit Centre, Khlong Nueng, Khlong Luang, 12120, Pathum Thani, Thailand.
Sci Rep. 2022 Apr 29;12(1):6995. doi: 10.1038/s41598-022-11001-5.
The plant factory with artificial light (PFAL) is a novel cultivation system of agriculture technology for crop production under controlled-environment conditions. However, there are a number of issues relating to low quality of seed germination and seedling vigor that lead to decreased crop yields. The present study investigates the optimal KNO concentration for seed germination, and the influence of different light spectra on early plant growth in holy basil (Ocimum tenuiflorum) under a PFAL system. Experiment 1 investigated the effects of KNO concentration (0, 0.2, 0.4 and 0.6%) on germination of seeds primed for 24 h under white Light emitting diodes (LED). Results show that sowing holy basil seeds in 0.4% KNO enhanced seed germination percentage (GP) and germination index (GI), while decreasing mean germination time (MGT). Experiment 2 investigated the effect of four light spectra on seed germination and early plant growth by sowing with 0 and 0.4% KNO and germinating for 15 days continuously under different monochromatic light settings: white, red, green and blue in PFAL. It was found that the green spectrum positively affected shoot and root length, and also decreased shortened MGT at 0 and 0.4% KNO when compared with other light treatments. Additionally, pre-cultivated seedlings under the green spectrum showed significant improvement in the early plant growth for all holy basil varieties at 15 days after transplanting by promoting stem length, stem diameter, plant width, fresh weights of shoot and root, and dry weights of shoot and root. These findings could be useful in developing seed priming and light treatments to enhance seed germination and seedling quality of holy basil resulting in increased crop production under PFAL.
人工光植物工厂（PFAL）是一种在可控环境条件下进行作物生产的新型农业技术种植系统。然而，存在一些与种子发芽质量和幼苗活力低有关的问题，导致作物产量下降。本研究探讨了 PFAL 系统中圣罗勒（Ocimum tenuiflorum）种子发芽的最佳 KNO 浓度，以及不同光质对早期植物生长的影响。实验 1 研究了在白光发光二极管（LED）下预浸泡 24 小时的种子在不同 KNO 浓度（0、0.2、0.4 和 0.6%）下的发芽效果。结果表明，在 0.4% KNO 中播种圣罗勒种子可提高种子发芽率（GP）和发芽指数（GI），同时降低平均发芽时间（MGT）。实验 2 通过在 PFAL 中不同单色光设置下（白光、红光、绿光和蓝光）播种 0 和 0.4% KNO 并连续发芽 15 天，研究了四种光质对种子发芽和早期植物生长的影响。结果表明，与其他光处理相比，绿光谱可积极影响茎和根的长度，同时降低在 0 和 0.4% KNO 下缩短的 MGT。此外，与其他光处理相比，在绿色光下预培养的幼苗在移栽后 15 天对所有圣罗勒品种的早期植物生长都有显著的促进作用，表现在茎长、茎直径、植株宽度、地上部和根鲜重、地上部和根干重的增加。这些发现对于开发种子预处理和光照处理以提高圣罗勒种子发芽率和幼苗质量，从而增加 PFAL 下的作物产量具有重要意义。
