Women's Lifecare Medicine, Obstetrics and Gynecology, School of Medicine, Osaka City University, Osaka, 545-8585, Japan.
Reproductive Science, Graduate School of Medicine, Osaka City University, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585, Japan.
Sci Rep. 2022 Apr 29;12(1):7064. doi: 10.1038/s41598-022-11251-3.
We assessed the effects of feeding regimen (ad libitum vs. time-restricted food access) and type of food (normal chow (NC: 12% fat) vs. moderately high calorie diet (mHCD: 31% fat)) on fertility competence of female mice. Mice fed mHCD had higher number of oocytes than mice fed NC. On the other hand, when mice were fed NC under time-restricted access to food (NT), the developmental rate to the blastocyst per number of normally fertilized ova was significantly decreased compared to others. The reactive oxygen species (ROS) level in oocytes increased in time-restricted food access and NC group. Transcriptome analysis of whole ovarian tissues from these mice showed a change in the cholesterol metabolism among the four groups. Time-restricted food access decreased serum LDL cholesterol level in both NC and mHCD groups. Moreover, the number of atretic follicles increased in NT mice compared to ad libitum food access mice. The present study shows that mHCD feeding increases the number of ovulated oocytes and that time-restricted feeding of NC impairs the developmental competence of oocytes after fertilization, probably due to the changes in serum cholesterol levels and an increase in the ROS content in oocytes.
我们评估了喂养方案（随意进食与限时进食）和食物类型（正常饲料（NC：12%脂肪）与中高热量饮食（mHCD：31%脂肪））对雌性小鼠生育能力的影响。与喂食 NC 的小鼠相比，喂食 mHCD 的小鼠的卵母细胞数量更多。另一方面，当喂食 NC 的小鼠限时进食（NT）时，与其他组相比，正常受精卵的囊胚发育率显著降低。在限时进食和 NC 组中，卵母细胞中的活性氧（ROS）水平增加。对这些小鼠的整个卵巢组织进行的转录组分析显示，四个组之间的胆固醇代谢发生了变化。限时进食降低了 NC 和 mHCD 两组的血清 LDL 胆固醇水平。此外，与随意进食的小鼠相比，NT 小鼠的闭锁卵泡数量增加。本研究表明，mHCD 喂养会增加排卵卵母细胞的数量，而 NC 的限时进食会损害受精后卵母细胞的发育能力，这可能是由于血清胆固醇水平的变化和卵母细胞中 ROS 含量的增加所致。
