科罗拉多州后院鸡（Gallus gallus domesticus）生化参考值的建立。
Establishment of biochemical reference values for backyard chickens in Colorado (Gallus gallus domesticus).
机构信息
Department of Microbiology, Immunology and Pathology, Colorado State University, Colorado, USA.
Veterinary Initiative for Endangered Wildlife (VIEW), Bozeman, Montana, USA.
出版信息
Vet Clin Pathol. 2022 Dec;51(4):577-584. doi: 10.1111/vcp.13136. Epub 2022 Apr 29.
BACKGROUND
Previous literature pertaining to biochemical RIs of domestic chickens has primarily focused on commercial production flocks and not backyard birds.
OBJECTIVE
We aimed to establish biochemistry RIs for privately-owned backyard chickens (Gallus gallus domesticus) using reference laboratory equipment.
METHODS
Samples were collected from 123 presumably healthy adult chickens between 2017 and 2019 from 22 different flocks in Colorado. Heparinized blood was obtained, and a biochemistry profile was evaluated, including sodium, potassium, chloride, calcium, phosphorous, uric acid, AST, CK, glucose, cholesterol, and total protein. Reference values were created according to current American Society for Veterinary Clinical Pathology recommendations.
RESULTS
Differences in measurand intervals compared with previous literature were found for sodium, calcium, total protein, potassium, phosphorus, uric acid, and glucose. Hens were found to have higher median calcium (17.9 mg/dL vs 11.2 mg/dL [P = .0001]), total protein (5.2 g/dL vs 4.8 g/dL [P = .0046]), and potassium (3.80 mEq/L vs 3.48 mEq/L [P = .0267]) concentrations, as well as lower sodium (155 mEq/L vs 158 mEq/L [P = .0046]) concentrations, calculated osmolalities (310 mOsm/kg vs 314 mOsm/kg [P = .0249]), and AST (165 U/L vs 194 U/L [P = .0121]) activities, than roosters. Seasonal variation was found between summer and winter samples for median sodium (144 mEq/L vs 148 mEq/L [P = .0008]), chloride (111.8 mEq/L vs 113.5 mEq/L [P = .0033]) concentrations, calculated osmolalities (306 mOsm/kg vs 311 mOsm/kg [P = <.0001]), and AST (185 U/L vs 159 U/L [P = .0053]) and CK (1098 U/L vs 770 U/L [P = .0007]) activities.
CONCLUSIONS
This study presents biochemical reference values for backyard chickens in Colorado that can be a basis for evaluations in similar settings.
背景
先前有关国内鸡生物化学参考区间（RI）的文献主要集中在商业生产鸡群，而不是后院鸡。
目的
我们旨在使用参考实验室设备为私人后院鸡（Gallus gallus domesticus）建立生物化学 RI。
方法
2017 年至 2019 年，从科罗拉多州的 22 个不同鸡群中采集了 123 只疑似健康成年鸡的肝素化血液样本。评估了生化分析，包括钠、钾、氯、钙、磷、尿酸、AST、CK、葡萄糖、胆固醇和总蛋白。根据当前美国兽医临床病理学会的建议制定参考值。
结果
与先前的文献相比，发现了钠、钙、总蛋白、钾、磷、尿酸和葡萄糖的测量区间差异。母鸡的钙（17.9mg/dL 比 11.2mg/dL [P = .0001]）、总蛋白（5.2g/dL 比 4.8g/dL [P = .0046]）和钾（3.80mEq/L 比 3.48mEq/L [P = .0267]）浓度较高，钠（155mEq/L 比 158mEq/L [P = .0046]）浓度、计算渗透压（310mOsm/kg 比 314mOsm/kg [P = .0249]）和 AST（165U/L 比 194U/L [P = .0121]）活性较低。与公鸡相比，母鸡的季节变化在夏季和冬季样本之间表现为钠（144mEq/L 比 148mEq/L [P = .0008]）、氯（111.8mEq/L 比 113.5mEq/L [P = .0033]）浓度、计算渗透压（306mOsm/kg 比 311mOsm/kg [P < .0001]）和 AST（185U/L 比 159U/L [P = .0053]）和 CK（1098U/L 比 770U/L [P = .0007]）活性。
结论
本研究提出了科罗拉多州后院鸡的生化参考值，可为类似环境下的评估提供依据。
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