Zlobina E N, Baryshnikov A Iu
Biull Eksp Biol Med. 1987 Mar;103(3):340-2.
M+-cell subpopulation forming M-rosettes and M(-)-cell subpopulation not forming M-rosettes were revealed in the peripheral blood of patients with B-cell chronic lympholeukemia (B-CLL) by means of mouse red blood rosette formation test. M+-subpopulation contained a larger percentage of cells expressing Ia-like antigens, as compared to M- subpopulation. On the other hand, the latter contained a significantly higher amount of cytoplasmatic immunoglobulin-containing cells. M+ appeared to be less mature than M- cells. Cells of B-CLL patients had a heterogeneous response to 12-0-tetradecanoylphorbol-13-acetate (TPA). Less mature cells with surface immunoglobulin expression did differentiate, while more mature cells containing cytoplasmatic immunoglobulins did not. Differentiation was accompanied by the acquisition of characteristics peculiar to more mature cells, i. e. cytoplasmatic immunoglobulin accumulation. Subpopulations of M+ and M- cells from each patient also had a different pattern of response to TPA: less mature M+ cells did differentiate, while more mature M- cells did not. Maturation of less mature leukemia cells, as the disease progresses, is suggested to result in a heterogeneous pattern of immunological B-CLL phenotype.
通过小鼠红细胞玫瑰花结形成试验，在B细胞慢性淋巴细胞白血病（B-CLL）患者的外周血中发现了形成M玫瑰花结的M +细胞亚群和不形成M玫瑰花结的M（-）细胞亚群。与M-亚群相比，M +亚群中表达Ia样抗原的细胞百分比更高。另一方面，后者含有明显更多的含细胞质免疫球蛋白的细胞。M +似乎比M-细胞成熟度更低。B-CLL患者的细胞对12-0-十四烷酰佛波醇-13-乙酸酯（TPA）有不同的反应。具有表面免疫球蛋白表达的较不成熟细胞确实发生了分化，而含有细胞质免疫球蛋白的较成熟细胞则没有。分化伴随着获得更成熟细胞特有的特征，即细胞质免疫球蛋白积累。来自每位患者的M +和M-细胞亚群对TPA也有不同的反应模式：较不成熟的M +细胞确实发生了分化，而较成熟的M-细胞则没有。随着疾病进展，较不成熟白血病细胞的成熟被认为导致B-CLL免疫表型的异质性模式。
