Department of Microbiology, Buller building, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry, University of Lethbridge, 4401 University Dr. W., Lethbridge, AB T1K 3M4, Canada.
Biotechnol Adv. 2022 Oct;59:107968. doi: 10.1016/j.biotechadv.2022.107968. Epub 2022 Apr 28.
Antimicrobial peptides (AMPs) found in a wide range of animal, insect, and plant species are host defense peptides forming an integral part of their innate immunity. Although the exact mode of action of some AMPs is yet to be deciphered, many exhibit membrane lytic activity or interact with intracellular targets. The ever-growing threat of antibiotic resistance has brought attention to research on AMPs to enhance their clinical use as a therapeutic alternative. AMPs have several advantages over antibiotics such as broad range of antimicrobial activities including anti-fungal, anti-viral and anti-bacterial, and have not reported to contribute to resistance development. Despite the numerous studies to develop efficient production methods for AMPs, limitations including low yield, degradation, and loss of activity persists in many recombinant approaches. In this review, we outline available approaches for AMP production and various expression systems used to achieve higher yield and quality. In addition, recent advances in recombinant strategies, suitable fusion protein partners, and other molecular engineering strategies for improved AMP production are surveyed.
抗菌肽 (AMPs) 广泛存在于动物、昆虫和植物物种中，是宿主防御肽的一部分，构成其先天免疫的重要组成部分。虽然某些 AMP 的确切作用模式尚未被破解，但许多 AMP 具有膜溶解活性或与细胞内靶标相互作用。抗生素耐药性的威胁日益严重，促使人们研究 AMP，以增强其作为治疗替代物的临床应用。与抗生素相比，AMP 具有许多优势，例如广泛的抗菌活性，包括抗真菌、抗病毒和抗细菌，并且尚未报道其有助于耐药性的发展。尽管有许多研究旨在开发有效的 AMP 生产方法，但许多重组方法仍然存在产量低、降解和失活等问题。在这篇综述中，我们概述了 AMP 生产的现有方法和各种表达系统，以实现更高的产量和质量。此外，还调查了重组策略、合适的融合蛋白伴侣和其他分子工程策略的最新进展，以提高 AMP 的生产。
