山兰旱稻抗旱相关长链非编码 RNA 研究。

Research on lncRNA related to drought resistance of Shanlan upland rice.

机构信息

Hainan Key Laboratory for Sustainable Utilization of Tropical Bio-Resources, College of Tropical Crops, Hainan University, Haikou, 570228, China.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

出版信息

BMC Genomics. 2022 Apr 30;23(1):336. doi: 10.1186/s12864-022-08546-0.

BACKGROUND

Drought has become the major abiotic stress that causes losses in rice yields and consequently is one of the main environmental factors threatening food security. Long non-coding RNA (lncRNA) is known to play an important role in plant response to drought stress, while the mechanisms of competing endogenous RNA (ceRNA) in drought resistance in upland rice have been rarely reported.

RESULTS

In our study, a total of 191 lncRNAs, 2115 mRNAs and 32 miRNAs (microRNAs) were found by strand-specific sequencing and small RNA sequencing to be differentially expressed in drought-stressed rice. Functional analysis of results indicate that they play important roles in hormone signal transduction, chlorophyll synthesis, protein synthesis and other pathways. Construction of a ceRNA network revealed that MSTRG.28732.3 may interact with miR171 in the chlorophyll biosynthesis pathway and affect the ability of plants to withstand drought stress by regulating Os02g0662700, Os02g0663100 and Os06g0105350. The accuracy of the regulatory network was verified by qRT-PCR.

CONCLUSION

Our results provide a theoretical basis for future studies on the potential function of lncRNA in plant drought resistance, and they provide new genetic resources for drought-resistant rice breeding.

背景

干旱已成为导致水稻减产的主要非生物胁迫因素，也是威胁粮食安全的主要环境因素之一。长链非编码 RNA（lncRNA）已被证实其在植物响应干旱胁迫中发挥着重要作用，而关于旱地水稻中竞争内源性 RNA（ceRNA）在抗旱性中的作用机制却鲜有报道。

结果

在我们的研究中，通过链特异性测序和小 RNA 测序，共发现了 191 个 lncRNA、2115 个 mRNAs 和 32 个 miRNAs（microRNAs）在干旱胁迫下的水稻中呈现差异表达。功能分析结果表明，它们在激素信号转导、叶绿素合成、蛋白质合成等途径中发挥重要作用。ceRNA 网络的构建表明，MSTRG.28732.3 可能与叶绿素生物合成途径中的 miR171 相互作用，并通过调控 Os02g0662700、Os02g0663100 和 Os06g0105350 来影响植物的抗旱能力。qRT-PCR 验证了调控网络的准确性。