Badhusha Allahbagash, Nafeez Ahmed Abdul, Suryakodi Selvam, Abdul Wazith Mohamed Jaffer, Mithra Sivaraj, Kanimozhi Kumarasamy, Abdul Majeed Seepoo, Taju Gani, Sahul Hameed Azeez Sait
Aquatic Animal Health Laboratory, C. Abdul Hakeem College (Affiliated to Thiruvalluvar University), Ranipet District, India.
J Fish Dis. 2022 Aug;45(8):1087-1098. doi: 10.1111/jfd.13631. Epub 2022 May 1.
This study reports the occurrence of cyprinid herpesvirus 3 (CyHV-3) in koi carp (Cyprinus carpio koi) for the first time in India. The koi carp, with clinical signs of ulcer with haemorrhage on body surface, necrosis of fin and discolouration of gill associated with huge mortality, were observed in aquarium shops, rearing tanks and grow-out ponds located in Chennai, India. The PCR assay carried out on infected fish samples using different primer sets specific to CyHV-3 confirmed its presence in the infected fish. Sequence analysis of partial thymidine kinase gene revealed 100% similarity with the sequence of CyHV-3 available in GenBank. Cell lines of koi carp and catla were found to be susceptible to CyHV-3 and its replication was confirmed by viral-specific cytopathic effect, PCR and bioassay. The CyHV-3 infection was reproduced by intramuscular injection of inoculum prepared from CyHV-3-infected fish to satisfy Koch's postulates. Tissue tropism of CyHV-3 in infected fish by PCR assay revealed the presence of CyHV-3 in all vital organs with prominent band in gill and gut tissue.
本研究首次报道了鲤疱疹病毒3型（CyHV-3）在印度锦鲤（Cyprinus carpio koi）中的出现。在印度钦奈的水族馆商店、养殖池和成鱼养殖池塘中，观察到体表有溃疡并伴有出血、鳍坏死和鳃变色等临床症状且死亡率极高的锦鲤。使用针对CyHV-3的不同引物对感染鱼样本进行PCR检测，证实了其在感染鱼中的存在。部分胸苷激酶基因的序列分析显示与GenBank中可用的CyHV-3序列具有100%的相似性。发现锦鲤和印度鲶的细胞系对CyHV-3敏感，其复制通过病毒特异性细胞病变效应、PCR和生物测定得到证实。通过肌肉注射由CyHV-3感染鱼制备的接种物来再现CyHV-3感染，以满足科赫法则。通过PCR检测CyHV-3在感染鱼中的组织嗜性，结果显示在所有重要器官中都存在CyHV-3，鳃和肠道组织中的条带尤为明显。
