College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China.
Colloids Surf B Biointerfaces. 2022 Jul;215:112521. doi: 10.1016/j.colsurfb.2022.112521. Epub 2022 Apr 26.
To improve the bioavailability of curcumin, surfactin was used to prepare curcumin-loaded nanoemulsions (Cur-NEs). Moreover, the physicochemical properties, digestive characteristics, as well as inhibition activity to Caco-2 cells of Cur-NEs were measured. Furthermore, the morphological analysis revealed that Cur-NEs with 320 mg/L surfactin appeared spherical nanoparticale (23.23 ± 2.86 nm) and uniform distribution. The encapsulation efficiency of Cur-NEs with 320 mg/L surfactin was 97.25 ± 1.28%. Simulated gastrointestinal digestion results indicated that surfactin elevated the sustained-release characteristics and higher bioaccessibility (40.92 ± 2.84%) of curcumin. Besides, Cur-NEs with 320 mg/L surfactin exhibited excellent stability in different temperature, pH and light irradiation. In addition, the inhibition of Cur-NEs with 320 mg/L surfactin to Caco-2 cells was 71.29%. Biochemical analysis showed that Cur-NEs enhanced the activity of lactate dehydrogenase, superoxide dismutase, catalase and glutathione peroxidase, as well as the reactive oxygen species content. RT-PCR and ELISA results also revealed that Cur-NEs inhibited Caco-2 cells through the activated mitochondria-mediated pathway. This study provided a strategy to encapsulate curcumin in nanoparticles with surfactin for improving bioavailability.
为了提高姜黄素的生物利用度，使用表面活性剂制备了载姜黄素的纳米乳（Cur-NEs）。此外，还测量了 Cur-NEs 的物理化学性质、消化特性以及对 Caco-2 细胞的抑制活性。此外，形态分析表明，表面活性剂浓度为 320mg/L 的 Cur-NEs 呈现出球形纳米颗粒（23.23±2.86nm）和均匀的分布。表面活性剂浓度为 320mg/L 的 Cur-NEs 的包封效率为 97.25±1.28%。模拟胃肠道消化结果表明，表面活性剂提高了姜黄素的持续释放特性和更高的生物可及性（40.92±2.84%）。此外，表面活性剂浓度为 320mg/L 的 Cur-NEs 在不同温度、pH 值和光照下表现出优异的稳定性。此外，表面活性剂浓度为 320mg/L 的 Cur-NEs 对 Caco-2 细胞的抑制率为 71.29%。生化分析表明，Cur-NEs 增强了乳酸脱氢酶、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性以及活性氧物质的含量。RT-PCR 和 ELISA 结果还表明，Cur-NEs 通过激活线粒体介导的途径抑制 Caco-2 细胞。本研究提供了一种用表面活性剂将姜黄素封装在纳米颗粒中的策略，以提高其生物利用度。
