Department of Pathology, National Cancer Institute, Cairo Univ. Egypt.
Genetic Engineering and Biotechnology Research Institute, Department of Molecular Diagnostics & therapeutics, University of Sadat City, Egypt.
Int J Immunopathol Pharmacol. 2023 Jan-Dec;37:3946320231176396. doi: 10.1177/03946320231176396.
Curcumin has antioxidant and antiproliferative properties, and its therapeutic effect must be considered. Nanocurcumin capsules showed a potential increase against biological cancer. This study sought to determine how curcumin nanoparticles and nanocapsules affected the expression of p53, Bcl-2, Bax, and Bax in a liver cancer cell line (Hep-G2). Mechanisms of apoptosis were also examined in this cell line.
This study used quantitative real-time polymerase chain reaction (qRT-PCR) to analyze the p53, Bcl-2, Bax, and Caspase-3 gene pathways and to evaluate the molecular mechanisms responsible for the efficacy of curcumin nanoparticles (CNPs) and curcumin nanocapsules (CNCs) against liver cell lines. Flow cytometry was used to check for signs of apoptosis and the cell cycle.
Curcumin nanocapsules produced by the ball milling process at 90 min significantly boosted the populations of apoptotic cells in a dose- and time-dependent manner. The mRNA expression analysis revealed that the proapoptotic Bax, Caspase-3, and the tumor suppressor gene p53 were upregulated throughout the process started by curcumin nanocapsules and decreased in the Bcl-2/Bax ratio
This research provides a fresh understanding of the molecular mechanisms behind the liver cancer-fighting abilities of curcumin nanoparticles. Curcumin nanocapsules produced through a unique mechanical technique can be used as an anticancer agent.
姜黄素具有抗氧化和抗增殖作用，其治疗效果必须考虑在内。纳米姜黄素胶囊显示出对生物癌症的潜在增加作用。本研究旨在确定姜黄素纳米粒子和纳米胶囊如何影响肝癌细胞系（Hep-G2）中 p53、Bcl-2、Bax 和 Bax 的表达。本研究还研究了该细胞系中的细胞凋亡机制。
本研究使用定量实时聚合酶链反应（qRT-PCR）分析 p53、Bcl-2、Bax 和 Caspase-3 基因途径，并评估姜黄素纳米粒子（CNPs）和姜黄素纳米胶囊（CNCs）对肝癌细胞系疗效的分子机制。流式细胞术用于检查细胞凋亡和细胞周期的迹象。
在 90 分钟的球磨过程中产生的姜黄素纳米胶囊以剂量和时间依赖的方式显著增加了凋亡细胞的群体。mRNA 表达分析显示，促凋亡 Bax、Caspase-3 和肿瘤抑制基因 p53 在姜黄素纳米胶囊启动的整个过程中均上调，而 Bcl-2/Bax 比值降低。
本研究为姜黄素纳米粒子抗癌能力背后的分子机制提供了新的认识。通过独特的机械技术生产的姜黄素纳米胶囊可用作抗癌剂。
