The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, 21287, USA.
Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, 21205, USA.
Exp Eye Res. 2022 Jul;220:109093. doi: 10.1016/j.exer.2022.109093. Epub 2022 Apr 28.
The purpose of this study was to evaluate the effect of bovine colostrum (BC) in the regeneration of corneal epithelial cells on an ocular alkali burn model. Twenty-four C57BL/6 mice were categorized into two gender/age-matched groups for treatment. Two days after inducing a corneal alkali burn in all left eyes with 4 μl of sodium hydroxide 0.15 mol/l, both eyes of group 1 were treated with BC 4 times per day, and both eyes of group 2 were treated with isotonic saline solution (SS). The epithelial defect was photographed and measured by fluorescein staining on days two, four, seven, and ten. Ocular burn damage was assessed with a pre-established classification in clock hours from the limbus. After 10 days both eyes were processed, half of the group's corneas were assessed histopathologically, and the other half was used for pro/anti-inflammatory cytokine quantification using ELISA. BC treated (Group 1) corneas revealed significantly improved fluorescein staining score for limbal involvement when compared to SS treated (Group 2) corneas at days 4 (p = 0.013), 7 (p < 0.001), and 10 (p < 0.001), respectively. No differences were noted in limbal involvement at day 2 between the two groups (p > 0.99). The overall change (difference in slope) in fluorescein staining for limbal involvement between days 2 and 10 was -0.1669 (p = 0.006). Histologic examinations and cytokine measurements of group 2 demonstrated a strong inflammatory component compared to group 1. Our data indicates that topical application of BC facilitates corneal re-epithelialization and wound healing by suppressing the inflammatory process in an ocular alkali burn model.
本研究旨在评估牛初乳（BC）对眼部碱烧伤模型中角膜上皮细胞再生的作用。将 24 只 C57BL/6 小鼠分为两组，进行性别和年龄匹配，用于治疗。所有左眼均用 4μl 0.15mol/l 氢氧化钠诱导角膜碱烧伤 2 天后，第 1 组每天 4 次给予 BC 治疗，第 2 组每天 4 次给予等渗盐水（SS）治疗。第 2、4、7 和 10 天，通过荧光素染色拍摄和测量上皮缺损，并拍照测量。用预先建立的从角膜缘开始的时钟小时分类评估眼烧伤损伤。10 天后，处理双眼，每组一半的角膜进行组织病理学评估，另一半用于使用 ELISA 定量分析促炎/抗炎细胞因子。与 SS 治疗（第 2 组）的角膜相比，BC 治疗（第 1 组）的角膜在第 4 天（p=0.013）、第 7 天（p<0.001）和第 10 天（p<0.001）时，在涉及角膜缘的荧光素染色评分上有显著改善。两组在第 2 天时在涉及角膜缘的荧光素染色评分上没有差异（p>0.99）。第 2 天和第 10 天之间涉及角膜缘的荧光素染色评分的总体变化（斜率差异）为-0.1669（p=0.006）。与第 1 组相比，第 2 组的组织学检查和细胞因子测量显示出强烈的炎症成分。我们的数据表明，在眼部碱烧伤模型中，BC 的局部应用通过抑制炎症过程，促进角膜再上皮化和伤口愈合。
