长链非编码 RNA PART1 通过 miR-374b/SALL1 轴抑制神经胶质瘤的增殖和迁移。
LncRNA PART1 inhibits glioma proliferation and migration via miR-374b/SALL1 axis.
机构信息
Department of Neurosurgery, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, 410005, Hunan Province, PR China.
Department of Neurosurgery, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha, 410005, Hunan Province, PR China.
出版信息
Neurochem Int. 2022 Jul;157:105347. doi: 10.1016/j.neuint.2022.105347. Epub 2022 Apr 29.
BACKGROUND
Abnormal expression of lncRNA is involved in a diversity of diseases and plays a vital role in targeted therapy. However, few studies have been conducted on lncRNA PART1 in glioma. We aimed to investigate the function and the potential regulatory mechanism of lncRNA PART1/miR-374b/SALL1 axis in glioma.
METHODS
qRT-PCR and western blotting detected genes and proteins expression. Dual-luciferase reporter assay was performed to examine the binding relationship of lncRNA PART1, miR-374b, and SALL1. MTT assay and clone formation assay were performed to detect the cell viability and proliferation. Transwell assay detected glioma cell migration. In vivo tumor development experiments detected changes in tumor size, volume, and weight of the tumor after overexpression of lncRNA PART1. Immunohistochemistry was used to detect ki-67, E-cadherin, and N-cadherin expression.
RESULTS
The expression of lncRNA PART1 and SALL1 were down-regulated and miR-374b was up-regulated in different glioma cell lines. Overexpression of lncRNA PART1 inhibited glioma cell proliferation, migration, and epithelial mesenchymal transition (EMT). LncRNA PART1 targeted miR-374b to promote SALL1 expression. The knockdown of miR-374b inhibited glioma cell proliferation and migration and EMT by SALL1. What's more, overexpression of miR-374b or knockdown of SALL1 reversed the inhibitory effect of lncRNA PART1 on the proliferation, migration, and EMT of glioma cells. Furthermore, overexpression of lncRNA PART1 inhibited glioma growth in vivo.
CONCLUSION
LncRNA PART1 inhibited glioma proliferation and migration via miR-374b/SALL1 axis. These results might provide new insights for comprehending the complex lncRNA-miRNA network in gliomas.
背景
长链非编码 RNA（lncRNA）的异常表达与多种疾病有关，在靶向治疗中起着至关重要的作用。然而，目前关于长链非编码 RNA PART1 在神经胶质瘤中的研究甚少。本研究旨在探讨 lncRNA PART1/miR-374b/SALL1 轴在神经胶质瘤中的功能及其潜在调控机制。
方法
qRT-PCR 和 Western blot 检测基因和蛋白表达。双荧光素酶报告基因实验检测 lncRNA PART1、miR-374b 和 SALL1 之间的结合关系。MTT 实验和集落形成实验检测细胞活力和增殖。Transwell 实验检测神经胶质瘤细胞迁移。体内肿瘤发展实验检测过表达 lncRNA PART1 后肿瘤大小、体积和重量的变化。免疫组化检测 ki-67、E-钙黏蛋白和 N-钙黏蛋白的表达。
结果
不同神经胶质瘤细胞系中 lncRNA PART1 和 SALL1 的表达下调，miR-374b 表达上调。过表达 lncRNA PART1 抑制神经胶质瘤细胞增殖、迁移和上皮间质转化（EMT）。lncRNA PART1 靶向 miR-374b 促进 SALL1 表达。miR-374b 敲低通过 SALL1 抑制神经胶质瘤细胞增殖、迁移和 EMT。此外，过表达 miR-374b 或敲低 SALL1 逆转了 lncRNA PART1 对神经胶质瘤细胞增殖、迁移和 EMT 的抑制作用。进一步的，过表达 lncRNA PART1 抑制了神经胶质瘤在体内的生长。
结论
lncRNA PART1 通过 miR-374b/SALL1 轴抑制神经胶质瘤的增殖和迁移。这些结果可能为理解神经胶质瘤中复杂的 lncRNA-miRNA 网络提供新的见解。
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