Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, 350116, China.
Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, 350116, China.
Anal Chim Acta. 2022 May 15;1207:339742. doi: 10.1016/j.aca.2022.339742. Epub 2022 Mar 19.
The availability of sensing platforms able to rapidly measure abused drugs directly in biological fluids in a single step would allow performing drugged driving screening on the site. The achievement of this goal is extremely important for preventing and controlling drug abuse and crime incidence. Motived by this, we constructed a simple, cost-effective and reagentless electrochemical aptamer-based (EAB) sensor with methamphetamine (MAMP) as the target molecule. This EAB sensor produced a nanomolar level of detection accuracy in unprocessed or minimally processed bio-samples. Specifically, circular dichroic spectrum was used to confirm that the truncated aptamer from the original sequence would undergo large binding-induced conformational changes. We then engineered the aptamer to work in the EAB platform and the resulting sensor enabled sensitive and specific detection of MAMP with the detection limit of 30 nM in undiluted serum, 50 nM in undiluted urine and 20 nM in 50% saliva. The sensor has good recovery rate, implying this method has good reliability and repeatability. The detection limit is far below the clinical detection threshold, it would be hopefully used for preliminary screening of drugged driving in real world.
能够在单一步骤中快速直接地在生物流体中测量被滥用药物的传感平台的可用性将允许在现场进行药物驾驶筛查。实现这一目标对于预防和控制药物滥用和犯罪发生率极为重要。受此启发，我们构建了一种简单、经济高效且无需试剂的基于适配体的（EAB）电化学传感器，以甲基苯丙胺（MAMP）为目标分子。该 EAB 传感器在未处理或仅经过最低限度处理的生物样本中达到纳摩尔级别的检测精度。具体来说，圆二色光谱被用于确认原始序列的截断适配体会经历大的结合诱导构象变化。然后，我们对适配体进行工程改造，使其在 EAB 平台上工作，由此产生的传感器能够对 MAMP 进行灵敏且特异性的检测，在未稀释的血清中的检测限为 30 nM，在未稀释的尿液中的检测限为 50 nM，在 50%唾液中的检测限为 20 nM。该传感器具有良好的回收率，这意味着该方法具有良好的可靠性和可重复性。检测限远低于临床检测阈值，有望用于实际药物驾驶的初步筛查。
