Department of Endodontics, Institute of Clinical Dentistry, Faculty of Dentistry, University of Oslo, Box 1109 Blindern, 0317 Oslo, Norway.
School of Dentistry, Institute of Clinical Sciences, College of Medical and Dental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
Dent Mater. 2022 Jun;38(6):1044-1059. doi: 10.1016/j.dental.2022.04.013. Epub 2022 Apr 28.
Assess the biological and physicochemical properties of AH Plus, BioRoot RCS and Pulp Canal Sealer (PCS) leachates with and without chlorhexidine (CHX).
The sealers were studied in no contact and 1-minute contact with CHX. For biological properties (antibacterial activity and cytotoxicity), leachates were formed in saline of freshly mixed, 1-, 7- and 28 days set sealers. The antibacterial properties of sealer leachates were investigated for planktonic and biofilm growth of E. faecalis, S. mutans, S.epidermidis and S.aureus. The 3-(4,5 dimethylthiazolyl-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate murine fibroblast cell viability after exposure to the leachates. The physical properties (water uptake, sorption, solubility, porosity, surface characteristics) of sealers and the pH of the immersion liquid (saline or distilled water) were also assessed over a 28-days period.
CHX improved the antibacterial properties of the sealer leachates and reduced cell viability for all sealer leachates, except for freshly mixed PCS. BioRoot RCS leachates presented the highest antibacterial properties and cell viability with and without CHX contact. PCS was the material most affected by CHX in terms of physical properties, whereas for AH Plus, solubility was increased. CHX did not affect the physical properties of BioRoot RCS, except for solubility that was decreased. CHX contact did not change sealers' alkalinity in distilled water whereas it increased it for AH Plus and BioRoot RCS in saline.
CHX improved the antibacterial efficacy of sealer leachates and either compromised or did not affect cell viability. CHX affected to various extent sealers' physicochemical properties.
评估 AH Plus、BioRoot RCS 和 Pulp Canal Sealer（PCS）在有无氯己定（CHX）情况下的浸提液的生物学和物理化学特性。
研究人员在无接触和 1 分钟接触 CHX 的情况下对密封剂进行了研究。对于生物特性（抗菌活性和细胞毒性），在新鲜混合、1 天、7 天和 28 天设置的密封剂的盐水中形成浸提液。研究了密封剂浸提液对粪肠球菌、变形链球菌、表皮葡萄球菌和金黄色葡萄球菌浮游和生物膜生长的抗菌性能。3-(4,5 二甲基噻唑基-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法用于评估暴露于浸提液后的小鼠成纤维细胞活力。还评估了密封剂的物理特性（吸水率、吸附率、溶解度、孔隙率、表面特性）和浸提液（盐水或蒸馏水）的 pH 值，为期 28 天。
CHX 提高了密封剂浸提液的抗菌性能，并降低了所有密封剂浸提液的细胞活力，除了新鲜混合的 PCS 外。BioRoot RCS 浸提液具有最高的抗菌性能和细胞活力，无论是否接触 CHX。就物理性能而言，PCS 是受 CHX 影响最大的材料，而对于 AH Plus，溶解度增加。CHX 不影响 BioRoot RCS 的物理性能，除了溶解度降低。CHX 接触不会改变 AH Plus 和 BioRoot RCS 在蒸馏水中的碱性，但会增加它们在盐水中的碱性。
CHX 提高了密封剂浸提液的抗菌功效，无论是损害还是不影响细胞活力。CHX 以不同程度影响密封剂的物理化学特性。
